Study on the biosynthesis and structure characterization of exopolysaccharide from Lactobacillus fermentum MC3

Trần Thị Ái Luyến, Đỗ Thị Bích Thủy, Trần Thị Văn Thi, Phan Thị Thu Huyền

DOI: http://dx.doi.org/10.26459/hueuni-jns.v127i1D.4943

Abstract


Abstract: The effects of carbohydrate sources in various concentration (2%, 3%, 4%, 5%, 6%) and fermentation conditions (such as initial cell density, temperature, pH and incubation time) on EPS synthesis of Lactobacillus fermentum MC3 were also studied. The results showed that adding different sugars (including glucose, lactose and sucrose) to culture medium significantly increased the EPS production. In comparison with other concentrations, EPS amounts were maximized in the medium supplemented with 4% (w/v) of sugars. The outcome was the highest for glucose, which was 178.207 mg/L, the obtained figures for lactose and for sucrose were 148.614 mg/L and 152.272 mg/L respectively. The results indicated that EPS production by L. fermentum MC3 reached the maximum values in the medium supplemented with 4% (w/v) glucose at 400C, pH 6.0, initial cell density of 106CFU/ml for 48 h cultivation with amount of 200.728 mg/L. By methylation analysis and gas–liquid chromatography–mass spectrometry (GLC–MS), the exopolysaccharide was found to be composed of D-mannose: D-glucose: D-galactose in a molar ratio of 1 : 0.74 : 0.09.


Keywords


Glucose, exopolysaccharide, Lactobacillus fermentum, LAB, methylation analysis

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