Effects of culture media on growth ability of Eurycoma longifolia Jack callus
Vol. 128 No. 1E (2019), Special Issues - Conference
Vol. 128 No. 1E (2019)

Effects of culture media on growth ability of Eurycoma longifolia Jack callus

Special Issues - Conference
https://doi.org/10.26459/hueuni-jns.v128i1E.5451
Published 2019-10-25
Nguyễn Hữu Nhân
Trường Cao đẳng Lương thực – Thực phẩm, 101B Lê Hữu Trác, Đà Nẵng, Việt Nam
Hoàng Tấn Quảng
Viện Công nghệ sinh học, Đại học Huế, Phú Thượng, Phú Vang, Thừa Thiên Huế, Việt Nam
Nguyễn Hoàng Lộc
Khoa Sinh học, Trường Đại học Khoa học, Đại học Huế, 77 Nguyễn Huệ, Huế, Việt Nam
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How to Cite

1.
Nhân NH, Quảng HT, Lộc NH. Effects of culture media on growth ability of Eurycoma longifolia Jack callus. hueuni-jns [Internet]. 2019Oct.25 [cited 2024Apr.23];128(1E):69-76. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5451
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Abstract

Eurycoma longifolia Jack (Simaroubaceae), one of the most popular tropical medicinal plants in South-east Asia. In this study, we investigated the growth ability of E. longifolia callus on various culture media to produce materials for later eurycomanone production. The results show that the combination of plant growth regulators was more effective than individuals. MS basal medium supplemented with 1.5 mg/L naphthaleneacetic acid and 1.0 mg/L kinetin had the best results. Callus grew strongly with a growth index of 11.24, fresh weight up to 32.92 g/flack, corresponding to a dry weight of 1.76 g/flack. HPLC analysis showed that callus extract had a peak with the same retention time as that of the eurycomanone standard and natural sample (4.15 min) with the eurycomanone content of 0.17 mg/g dry weight.

https://doi.org/10.26459/hueuni-jns.v128i1E.5451
PDF (Vietnamese)

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