TY - JOUR AU - Hoàng, Tấn Quảng AU - Phạm, Thị Diễm Thi AU - Lan, Trần Thúy AU - Phạm, Mai Thu Thủy AU - Vũ, Đức Hoang AU - Phan, Thị Á Kim PY - 2021/09/30 Y2 - 2024/03/29 TI - Effects of culture medium on callus growth of Gynostemma pentaphyllum (Thunb.) Makino – a valuable medicinal plant JF - Hue University Journal of Science: Natural Science JA - hueuni-jns VL - 130 IS - 1C SE - DO - 10.26459/hueunijns.v130i1C.6364 UR - https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/6364 SP - 127-137 AB - <p><em>Gynostemma pentaphyllum</em> (Thunb.) Makino (Jiaogulan) is a traditional medicinal herb belonging to the Cucurbitaceae family. <em>G. pentaphyllum</em> grows widely in Southern China, Japan, Korea, and Vietnam. The essential bioactive components of Jiaogulan are saponin glycosides (gypenosides) and antioxidants. Jiaogulan exhibits bioactive activities such as anticancer, antioxidant, cholesterol-reducing agent, immunopotentiation, and others. In this study, the primary callus of Jiaogulan was used as a material to evaluate the influence of the culture medium on the induction and growth of secondary calli. The results reveal that the callus cultured on the MS medium supplemented with 2.0 mg/L kinetin and 0.5 mg/L indole-3-butyric acid has the best growth ability, high rate of secondary callus induction (100%), and good callus quality for suspension culture. The concentration of gypenoside and Rb1 in callus is 36.298 and 0.009 mg/g dry weight. The gypenoside concentration of callus is lower than that of leaves (65.58%) and almost similar to that of stems (92.38%) from natural samples. The suitable solvent for the extraction of gypenoside is methanol. The obtained callus will be used as material for cell suspension culture in further studies.</p> ER -