GENETIC DIVERSITY OF ANOECTOCHILUS ROXBURGHII IN HUE CITY, VIETNAM, BASED ON RAPD MARKERS

2025-03-25T07:33:54+00:00

Anoectochilus roxburghii (Wall.) Lindl, a valuable medicinal plant, is under threat due to overexploitation and adverse growing conditions. Therefore, genetic research is necessary to preserve and create hybrid varieties for breeding. In this study, we used RAPD markers to assess genetic differences among 17 A. roxburghii accessions collected in Hue City. The results showed that 17 RAPD selected primers were suitable for evaluating genetic polymorphism due to the high percentage of polymorphic bands (76.105%) and the n_a, n_e, h, and I indices gave high values, reaching 1.762, 1.428, 0.249 and 0.373, respectively. Among these 17 primers, UBC#405, UBC#427, UBC#446, UBC#469, UBC#476 and UBC#498 were the six most optimal primers for RAPD marker development with the highest PIC, Rp, MI, and EMR indices. Additionally, the UPGMA tree was separated into three clusters, which showed the genetic grouping among accessions in the population of A. roxburghii. The similarity coefficients ranged from 0.635 to 0.930. HUIB_AR10 was most similar to HUIB_AR06 while it was most different from HUIB_AR01.

MICROWAVE-ASSISTED EXTRACTION OF POLYPHENOL-RICH EXTRACT AND EVALUATION OF DPPH RADICAL SCAVENGING ACTIVITY FROM LEAVES OF VITEX NEGUNDO L. (VERBENACEAE)

2025-06-04T08:52:41+00:00

This research investigates the ultrasound-assisted extraction (UAE) of polyphenol-rich extracts from leaves of Vitex negundo L. (Verbenaceae) and evaluates their antioxidant activity. Dried leaf powder, cultivated in Ho Chi Minh City, Vietnam, was extracted using 60% ethanol under varying conditions of sample-to-solvent ratio, temperature, and time. Optimal extraction was achieved at a ratio of 1:10 (g/ml), 50°C, and 40 minutes, resulting in the highest total polyphenol (72.90 mg GAE/g) and flavonoid contents (179.94 mg QE/g). The final concentrated extract showed a moisture content of 4.90%, extraction yield of 27.94%, and retained 42.84 mg GAE/g of polyphenols and 165.67 mg QE/g of flavonoids. Antioxidant capacity was assessed via DPPH radical scavenging assay. The extract exhibited moderate activity with an IC₅₀ of 66.08 μg/ml, compared to 12.61 μg/ml for ascorbic acid and 4.48 μg/ml for BHA. The results demonstrate that V. negundo leaves are a promising source of natural antioxidants. UAE was shown to be an effective and green technique for enhancing extraction efficiency. These findings support the potential application of V. negundo leaf extract in functional foods and phytopharmaceuticals.

Hue University Journal of Science: Agriculture and Rural Development

GENETIC DIVERSITY OF ANOECTOCHILUS ROXBURGHII IN HUE CITY, VIETNAM, BASED ON RAPD MARKERS

MICROWAVE-ASSISTED EXTRACTION OF POLYPHENOL-RICH EXTRACT AND EVALUATION OF DPPH RADICAL SCAVENGING ACTIVITY FROM LEAVES OF VITEX NEGUNDO L. (VERBENACEAE)