@article{Nguyễn_Nguyễn_Trịnh_Lê_Lê_2022, title={Characterization of extracellular protease produced by Bacillus tequilensis ON1}, volume={131}, url={https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/6410}, DOI={10.26459/hueunijns.v131i1A.6410}, abstractNote={<p>A bacterial strain with casein hydrolytic activity was isolated from shrimp waste sludge from a culture pond at Phong Dien, Thua Thien Hue. Molecular identification using 16S rRNA nucleotide sequence shows a high similarity to <em>Bacillus tequilensis</em> on the NCBI database. Hence, the isolate was named <em>B. tequilensis</em> ON1. Extracellular protease activity value is 192,42 U·mL<sup>–1</sup> after 24 h culture. Zymogram SDS-PAGE electrophoresis shows that the major extracellular protease has a molecular weight of 130 kDa. The enzyme exhibits maximal activity at 50 °C and pH 8. Protease activity increases in the presence of Ca<sup>2+</sup> and Mg<sup>2+</sup> ions, while Mn<sup>2+</sup> inhibits protease activity. The protease activity was more active and stable in hydrophobic organic solvents.</p>}, number={1A}, journal={Hue University Journal of Science: Natural Science}, author={Nguyễn, Quang Lịch and Nguyễn, Đức Huy and Trịnh, Thị Phương Thảo and Lê, Thị Kim Thoa and Lê, Công Tuấn}, year={2022}, month={Mar.}, pages={109-117} }