TY - JOUR AU - Hồng, Hoàng Thị Kim AU - Ngọc Hạnh, Nguyễn Thị AU - Hoàng, Đinh Tiến AU - Long, Đặng Thanh AU - Minh Thu, Ngô Thị AU - Hà, Nguyễn Việt AU - Hồng Trang, Phạm Thị PY - 2019/10/25 Y2 - 2024/03/28 TI - Effects of water stress treatment on fluctuations of MtDHDPS1 gene encoding of dihydrodipicolinate synthase in Medicago truncatula JF - Hue University Journal of Science: Natural Science JA - hueuni-jns VL - 128 IS - 1E SE - DO - 10.26459/hueuni-jns.v128i1E.5434 UR - https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5434 SP - 27-37 AB - <p>This paper presents the results of the effect of water stress treatment on the fluctuation of the <em>MtDHDPS1</em> gene encoding dihydrodipicolinate synthase (MtDHDPS, EC 4.2.1.52) in leaves of <em>M. truncatula</em>. Enzyme MtDHDPS plays an important role in the regulation of lysine biosynthesis in the Legume group, in general, and <em>M. truncatula</em>, in particular. The 3-week-old plants were treated with water stress in shortage for 5 days and 10 days, then the samples were corrected for research. The results of quantitative mRNA analysis by RT-PCR technique show that the water stress treatment has changed the expression level of this gene, in which the threshold cycle value (Ct) of MtDHDPS1 gene in both 5-day and 10-day drought-treated samples is lower than the corresponding untreated control water samples, and the level of MtDHDPS1 gene expression in 5-day drought-treated plants is 2<sup>5.53 </sup>­­­­­= 46.2 times as high as that in the 10-day drought-treated plants.</p> ER -