APPLICATION OF SHIFTING ASSAY OF STANDARDIZED INDIRECT AGGLUTINATION (SSIA) FOR DETECTION OF ANTIGENS OF NEWCASTLE DISEASE AND INFECTIOUS BURSAL DISEASE VIRUSES IN CHICKEN FAECES

Authors

  • Phạm Hồng Sơn Khoa Chăn nuôi - Thú y, Đại học Nông Lâm
  • Phạm Hồng Kỳ
  • Nguyễn Thị Lan Hương
  • Phạm Thị Hồng Hà

Abstract

. Using the method of shifting assay of standardized indirect agglutination (SSIA), the prevalence of Newcastle disease viruses (NDV) and infectious bursal disease viruses (IBDV) in chickens reared in several districts of Thua Thien Hue province in the Spring-Summer and Fall-Winter seasons was determined. In the Spring-Summer season of 2011, about 22.3% of the chickens were infected with NDV, in which A Luoi  accounted for the highest percentage of 25% of the infected chickens and Huong Thuy  the lowest  of 18.2%. Meanwhile, 36% of the same chickens were infected with IBDV, with the highest percentage (46.66%) also in A Luoi and the lowest (30.3%) also in Huong Thuy. The intensity of NDV infection in the Spring-Summer season in A Luoi and Phu Vang was highest (GMT = 1.45), and in Huong Thuy lowest (GMT = 1.31). In addition, in the Fall-Winter season, about 46% of the chickens were infected with NDV and 46.3% with IBDV in Huong Thuy and Phu Vang – two neighbouring districts of Hue City, in which NDV was detected in 54.4% of the chickens in Huong Thuy and 33.9% in Phu Vang. In contrast, IBDV was detected in 41.9% and 52.7% of the chickens respectively in the two districts. The infection was not inter-dependent. Methodically, although the differences in the infection rates were insignificant with the accuracy of 95%, faecal samples showed higher sensitivity in SSIA analyses for both cases of NDV and IBDV infection in comparision with mouth exudates. By SSIA method, results could be read clearly with unaided eyes for a long time after the performance, and it was also proven applicable for cases of haemagglutinating viruses if proper treatments for depletion of animal RBCs’ surface agglutinins could be applied.

References

. C. W. Beard, R. P. Hanson, Newcastle Disease. In: Diseases of Poultry, 8th edition, M. S. Hofstad, H. J. Barnes, B. W. Calnek, W. M. Reid, H. W. Yoder (eds.). Iowa State University Press, Ames, Iowa, USA, (1984), 452-470.

. S. V. Boyden, The absorption of proteins on erythrocytes treated with tannic acid and subsequent haemagglutination by antiprotein sera, J. Exp. Med., No. 93 (1951), 107.

. N. F. Cheville, Studies on the pathogenesis of Gumboro disease in the bursa of Fabricius, spleen and thymus of the chicken, Am. J. Pathol., No. 51 (1967), 527-551.

. S. D. Cosgrove, An apparently new disease of chickens avian nephrosis, Avian Dis., No. 6, (1962), 385-389.

. Nguyen Tien Dzung, Bệnh Gumboro ở gà và tình hình dịch bệnh ở Việt Nam [The Gumboro infections in the chicken and the disease situation in Vietnam] (in Vietnamese), Tạp chí Khoa học Kỹ thuật Nông nghiệp, 2, (1989), 104-109.

. P. D. Lukert, Y. M. Saif, Infectious bursal disease. In: Disease of poultry, 10th edition, B. W. Calnek ed., Iowa State University Press, Ames, Iowa, USA, (1997), 721-738.

. Nguyen Thi Hoang Oanh, Pham Thi Hong Lam, Do Thi Loi, Pham Hong Son, Sử dụng tổ hợp phản ứng ngưng kết hồng cầu trực tiếp với trắc định xê lệch ngưng kết hồng cầu chuẩn (HA-SSDHA) và trắc định xê lệch ngưng kết gián tiếp chuẩn (SSIA) trong chẩn đoán bệnh Newcastle [Application of combination of direct haemagglutination with shifting assay of direct haemagglutination inhibition (HA-SSDHI) and shifting assay of indirect agglutination inhibition (SSIA) in diagnosis of Newcastle disease] (in Vietnamese), Tạp chí Khoa học Kỹ thuật Thú y, Vol XIX, No. 5, (2012), 48-56.

. Pham Hong Son, Tình hình bệnh dịch tả lợn qua chẩn đoán huyết thanh học tại Thừa Thiên Huế [Situation of classical swine fever in Thu Thien Hue studied with serological diagnoses] (in Vietnamese), Tạp chí Khoa học Kỹ thuật Thú y, Vol XI, No. 2, (2004), 11-18.

. Pham Hong Son, Nghiên cứu tạo kháng nguyên ngưng kết hồng cầu gián tiếp gắn virus cúm A và vận dụng mới trong chẩn đoán bệnh cúm ở gia cầm [Reseach on the formulation of Avian influenza A virus-coated indirect haemagglutination antigens and new application of them in diagnosis of the disease in chickens] (in Vietnamese), Tạp chí Khoa học Kỹ thuật Thú y, Vol XVI, No. 2, (2009), 14-22.

. G. W. Snedecor, W. G. Cochran (eds.), Statistical methods, 7th ed., Iowa State University Press, Ames, Iowa, USA, (1980), 121-124.

Published

2013-10-03