This study aims to isolate aerobic bacteria strains for decomposing chlorpyrifos in soil in Lam Dong. The number of microorganisms for decomposing chlorpyrifos can be increased by incubating the soil samples in MSM medium supplemented with chlorpyrifos (20 mg/L) as the only carbon source. Three aerobic bacteria strains were isolated after the separating each bacterial strain and investigating the decomposition ability chlorpyrifos processing, which were further named as T1, W3 and B2, respectively. In particular, T1, W3 and B2 strains removed 50.4%, 59.3% and 62.2% of chlorpyrifos after 14 days of culture in MSM medium in supplementation with 20 mg/L chlorpyrifos, respectively. Acinetobacter calcoaceticus, Bacillus megaterium and Sphingomonas pseudosanguinis were identified by 16S rRNA gene sequencing. The three strains of bacteria exhibit ability to degrade chlorpyrifos when added to the soil environment. These results suggested that the isolated bacteria strains can be applied to treat contaminated soil with pesticides and irritate the plant growing.