Keywords
lan Thiên nga đen
lát cắt giả hành
nhân chồi
tái sinh chồi Fredclakeara After Dark
pseudobulb slice
shoot multiplication
shoot regeneration
Abstract
Fredclarkeara After Dark is an artificial orchid species with black flowers. Micropropagation of Fredclarkeara After Dark allows the production of many plantlets with the same genetic characteristics as mother plants. This paper presents the results of in vitro shoot multiplication of Fredclarkeara After Dark from pseudobulbs and roots of one-year-old natural plants. The results indicate that highly effective sterilization occurs with 1% NaClO in five minutes in step 1 and seven minutes in step 2 with a root segments survival rate of 47.30%. The viability of pseudobulb slices after being immersed in 1% NaClO for 15 minutes in step 1 and 10 minutes in step 2 is 55,88%. The half-strength MS medium supplemented with 0.6 mg/L BAP is the optimal medium for shoot regeneration from roots, with an induction rate of 60.87% after 18.55 days. The half-strength MS medium supplemented with 0.4 mg/L BAP is suitable for shoot regeneration from pseudobulbs, with an induction rate of 60.87% after 18 days. The highest number of shoots per explant (2.15) was obtained on the ½ MS medium supplemented with 0.4 mg/L BAP after 16 weeks of culture. This medium provides the most roots at 3.95/sample, with an average root length of 2.96 cm.
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