Effects of mixed microorganisms cultures in controlling harmful insects on vegetables
Vol. 130 No. 3D (2021), Original Article
Vol. 130 No. 3D (2021)

Effects of mixed microorganisms cultures in controlling harmful insects on vegetables

Original Article
https://doi.org/10.26459/hueunijard.v130i3D.6177
Published 2021-11-04
Le Tat Dat
GREEN LAB – Center for Life Science Research (CELIFE) and Department of Microbiology, Faculty of Biology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan, Hanoi, Vietnam
Nguyen Thi Thu Thuy
GREEN LAB – Center for Life Science Research (CELIFE) and Department of Microbiology, Faculty of Biology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan, Hanoi, Vietnam
Nguyen Huu Hoan
GREEN LAB – Center for Life Science Research (CELIFE) and Department of Microbiology, Faculty of Biology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan, Hanoi, Vietnam
Pham Thi Det
Institute of Research and Application of Advanced Biotechnology (HIBIOTEK), 314 Minh Khai St., Hai Ba Trưng, Hanoi, Vietnam
Tran Thi Anh Dao
Bao Minh Chau Co. Ltd., 43/1 Bui Quang La St., Go Vap, Ho Chi Minh City, Vietnam
Van My Tien
Bao Minh Chau Co. Ltd., 43/1 Bui Quang La St., Go Vap, Ho Chi Minh City, Vietnam
Pham The Hai
GREEN LAB – Center for Life Science Research (CELIFE) and Department of Microbiology, Faculty of Biology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan, Hanoi, Vietnam
Nguyen Truong Giang
Institute of Research and Application of Advanced Biotechnology (HIBIOTEK), 314 Minh Khai St., Hai Ba Trưng, Hanoi, Vietnam
PDF (Vietnamese)

Keywords

harmful insects
microbial products for insect control
Helicoverpa armigera
Liriomyza sativae
Empoasca flavescens
mixed culture chế phẩm sinh học diệt côn trùng
hỗn hợp vi sinh vật
sâu hại rau
sâu xanh
giòi đục lá
rầy xanh

Abstract

In Vietnam, the safe production of vegetables is now a high demand and requires the use of biological agents to replace chemicals, especially for insect control. Microbial products for insect control, which have therefore far been developed, are mainly single-culture-based; hence, their efficacious spectrums are narrow. In this study, we created several mixed cultures of Bacillus thuringiensis strain PAM32 (Bt), Metarhizium anisopliae strain PAM23, and Beauveria bassiana strain PAM21 and investigated their potential in controlling some harmful insects on vegetables and their possible interactions in such activities. The 0.9% NaCl suspensions of the mixed cultures displayed high insecticidal efficacies and synergistic effects against Helicoverpa armigera (in the laboratory, causing an 85% death rate after three days) and Liriomyza sativae (in the field, reducing 70% of affected tomato leaves). The presence of Bt in the mixtures could enhance the insecticidal efficiency (up to 50%), even to Empoasca flavescens in the field test. The results indicate that using mixed microbial cultures can exploit synergistic interactions and complementary interactions among their individuals, thus improving the overall insecticidal effect.

https://doi.org/10.26459/hueunijard.v130i3D.6177
PDF (Vietnamese)

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