Antifungal ability of Aloe vera L. extract combined with nano chitosan against Aspergillus niger A2 in purple onion bulbs

Abstract

We examine the antifungal effect of the Aloe vera L. extract with nano chitosan on Aspergillus niger A2 infecting purple onion bulbs in in vitro and in vivo. The combination of Aloe vera L. extract and 0.1% nano chitosan exhibits higher inhibition on the fungus than nano chitosan alone. The higher the concentration of Aloe vera L. extract in the mixture, the higher the inhibition of the fungi. In vitro, the Aloe vera L. extract with a concentration of 10% in the mixture completely inhibits the growth of A. niger A2 on the PDA and PDB media. The inhibitory effect on the mycelial diameter and dry biomass is 50 and 90% for the 4.64 and 10.27% Aloe vera L. extract on the PDA media and 1.97 and 7.51% on the PDB media. In vivo, the concentration of 20% Aloe vera L. extract completely inhibits the growth of A. niger A2 on purple onion bulbs after 15 days at 28 °C. Meanwhile, the concentration of 10% Aloe vera L. extract inhibits 89.19% of the decay of purple onion bulbs with MIC₅₀ and MIC₉₀ at 3.19 and 10.64% Aloe vera L. extract.