In vitro propagation of Centella (Centella asiatica L.), in Quang Tho, Thua Thien Hue
Vol. 132 No. 3D (2023), Original Article
Vol. 132 No. 3D (2023)

In vitro propagation of Centella (Centella asiatica L.), in Quang Tho, Thua Thien Hue

Original Article
https://doi.org/10.26459/hueunijard.v132i3D.7204
Published 2023-12-21
Truong Thi Hong Hai
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
https://orcid.org/0000-0002-6591-5485
Pham Thi Diem Thi
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
Hoang Tan Quang
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
Nguyen Quang Co
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
PDF (Vietnamese)

Keywords

môi trường SH
nhân giống in vitro
nhân nhanh chồi
Quảng Thọ
rau má Centella
in vitro propagation
Quang Tho
SH medium
shoot multiplication

Abstract

In Quang Tho commune, Quang Dien district, Thua Thien Hue province, Centella is a primary product to increasing farmers’ income. To provide disease-free plantlets for organic cultivation, we developed a micropropagation method for this culture. The stem explants washed and sterilized with 0.1% HgCl2 for 8 min exhibit the highest survival rate of 66.67%. The SH salt medium supplemented with 30 g/L sucrose, 9 g/L agar, and 1 mg/L BAP is suitable for culturing stem segments, with 3.37 regenerated shoots per sample. The medium for shoot multiplication is SH supplemented with 2 mg/L BAP, 0.2 mg/L TDZ, and 1 mg/L KIN, providing 7.7 shoots per sample. The roots form and develop well on the ½ SH medium supplemented with 1 mg/L IBA, with 14.83 roots per shoot. The suitable substrate for in vitro plant growth is a mixture of 50% soil and 50% rice husk, with a 92.24% survival rate.

https://doi.org/10.26459/hueunijard.v132i3D.7204
PDF (Vietnamese)

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