In vitro propagation of Centella (Centella asiatica L.), in Quang Tho, Thua Thien Hue

Keywords

centella
in vitro propagation
Quang Tho
SH medium
shoot multiplication môi trường SH
nhân giống in vitro
nhân nhanh chồi
Quảng Thọ
rau má

Abstract

In Quang Tho commune, Quang Dien district, Thua Thien Hue province, Centella is a primary product to increasing farmers’ income. To provide disease-free plantlets for organic cultivation, we developed a micropropagation method for this culture. The stem explants washed and sterilized with 0.1% HgCl2 for 8 min exhibit the highest survival rate of 66.67%. The SH salt medium supplemented with 30 g/L sucrose, 9 g/L agar, and 1 mg/L BAP is suitable for culturing stem segments, with 3.37 regenerated shoots per sample. The medium for shoot multiplication is SH supplemented with 2 mg/L BAP, 0.2 mg/L TDZ, and 1 mg/L KIN, providing 7.7 shoots per sample. The roots form and develop well on the ½ SH medium supplemented with 1 mg/L IBA, with 14.83 roots per shoot. The suitable substrate for in vitro plant growth is a mixture of 50% soil and 50% rice husk, with a 92.24% survival rate.

https://doi.org/10.26459/hueunijard.v132i3D.7204

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