Evaluation of antagonistic ability against by colletotrichum fructicola CL5 causing anthracnose disease on chilli by β-1,3-glucanase producing bacillus spp.
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Colletotrichum fructicola
biocontrol β-1,3-glucanase
Colletotrichum fructicola,
đối kháng sinh học


β-1,3-glucanase is a crucial enzyme for the development of plants. It is involved in the maturation of seeds, floral development, and cell division. Furthermore, β-1,3-glucanase prevents the spreading of diseases by fungal pathogens to plants. This study chose two bacterial strains that produced β-1,3-glucanases, including Bacillus sp. 4 and Bacillus sp. 41 to attract fungus pathogen C. fructicola CL5 in chili inhibition ability. The results indicated Bacillus sp. 41. (secreted highest enzyme activity of 1,202± 0,04 U/mL after 12 hours of culture), higher than that of Bacillus sp. 4 (enzyme reached maximal of 0,85379 ± 0,04 U/mL after 15 hours of culture). The effect on C. fructicola CL5 growth prevention by the two Bacillus spp. in vitro models resulted in (Bacillus sp. 4: 50,73% after six days and Bacillus sp. 41: 57,39% after 12 days). In this result, β-1,3-glucanase was produced by Bacillus spp. positively contributed to the control of C. fructicola CL5 causing anthracnose disease in chili, which is equal to the commercial fungal pesticide Ridomil.

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  1. Kilani-Feki, O., Ben Khedher, S., Dammak, M., Kamoun, A., Jabnoun-Khiareddine, H., Daami-Remadi, M., Tounsi, S. (2016), Improvement of antifungal metabolites production by Bacillus subtilis V26 for biocontrol of tomato postharvest disease, Biological Control, (95), 73–82.
  2. Baysal, O., Caliscan, M., Yesilova, O. (2008), An inhibitory effect of a new Bacillus subtilis strain (EU07) against Fusarium oxysporum f. sp. Radicislycopersici, Physiological and Molecular Plant Pathology, 73(1–3), 25–32.
  3. Cui, L., Yang, C., Wei, L., Li, T., Chen, X. (2020), Isolation and identification of an endophytic bacteria Bacillus velezensis 8-4 exhibiting biocontrol activity against potato scab, Biological Control, (141), 104–156.
  4. Srividya, S., Sasirekha, B., Ashwini, N. (2012), Multifarious antagonistic potentials of rhizosphere associated bacterial isolates against soil borne diseases of Tomato, Asian Journal of Plant Science and Research, 2(2), 180–186.
  5. Leifertl, C., Li, H., Chidbureel, S., Hampson, S., Workman, S., Sigee, D. (1995), Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45, J Appl Bacteriol, 78(2), 97–108.
  6. Ahimou, F., Jacques, P., Deleu, M. (2000), Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity, Enzyme Microb. Technol., 27, 749–754.
  7. Bowman, S. (2006), Free The structure and synthesis of the fungal cell wall, BioEssays, 28(8), 799–808.
  8. Perrot, Thomas, Markus, P., Vicente, R. (2022), Emerging Roles of β-Glucanases in Plant Development and Adaptative Responses, Plants, 11(9), 1119.
  9. Sapirstein, H. (2016), Bioactive Compounds in Wheat Bran, Encyclopedia of Food Grains, 268–276.
  10. Wu, Q., Dou, X., Wang, Q., Guan, Z., Cai, Y., Liao, X. (2018), Isolation of β-1,3-Glucanase-Producing Microorganisms from Poria cocos Cultivation Soil via Molecular Biology, Molecules, 23(7), 1555.
  11. Burner, R. (1964), Determination of reducing sugar value 3,5-dinitrosalicylic acid method, Methods Carbohydr Chem, 4, 67–71.
  12. Wood, I., Elliston, A., Ryden, P., Bancroft, I., Roberts, I., Waldron, K. (2012), Rapid quantification of reducing sugars in biomass hydrolysates: Improving the speed and precision of the dinitrosalicylic acid assay, Biomass and Bioenergy, (44), 117–21.
  13. Bai, L., Kim, J., Son, K., Shin, D., Ku, B., Kim, D., Park, H. (2021), Novel Anti-Fungal D-Laminaripentaose-Releasing Endo-β-1,3-glucanase with a RICIN-like Domain from Cellulosimicrobium funkei HY-13, Biomolecules, 11(8), 1080.
  14. Kruijt, M., Tran, H., Raaijmakers, J. (2009), Functional, genetic and chemical characterization of biosurfactants produced by plant growth-promoting Pseudomonas putida 267, J. Appl. Microbiol., 107(2), 546–556.
  15. Li, X., Zhang, Y., Wei, Z., Guan, Z., Cai, Y., Liao, X. (2016), Antifungal Activity of Isolated Bacillus amyloliquefaciens SYBC H47 for the Biocontrol of Peach Gummosis, PLoS One, 11(9).
  16. Fan, H., Ru, J., Zhang, Y., Wang, Q., Li, Y. (2017), Fengycin produced by Bacillus subtilis 9407 plays a major role in the biocontrol of apple ring rot disease, Microbiological Research, 199, 89–97.
  17. Zhou, A., Wang, F., Yin, J., Peng, R., Deng, J., Shen, D., Wu, J., Liu, X., Ma, H. (2022), Antifungal action and induction of resistance by Bacillus sp. strain YYC 155 against Colletotrichum fructicola for control of anthracnose disease in Camellia oleifera, Front Microbiol., 25, 13, 956642. Bertram P., Chen P., Buller C., Akagi J., Endo-(1,3)-β-D-glucanase activity secreted by Bacillus sp., (1994), Letters in Applied Microbiology, 19(5), 349–352.
  18. Badiaa, E., Abdeljabbar, H., Mohamed, R. H., Abdellatif, B., Najla S. (2012), In vivo and in vitro evaluation of antifungal activities from a halotolerant Bacillus subtilis strain J9, African Journal of Microbiology Research, 6(19), 4073–4083.
  19. You, W., Ge, C., Jiang, Z., Chen, M., Li, W. (2021), Shao Y. Screening of a broad-spectrum antagonist-Bacillus siamensis, and its possible mechanisms to control postharvest disease in tropical fruits, Biological Control, 157.
  20. Prakash, J., Egamberdieva, D., Arora, N. (2022), A Novel Bacillus safensis-Based Formulation along with Mycorrhiza Inoculation for Controlling Alternaria alternata and Simultaneously Improving Growth, Nutrient Uptake, and Steviol Glycosides in Stevia rebaudiana under Field Conditions, Plants (Basel)., 11(14), 1857.
  21. Aiting, Z., Fang, W., Jiabi, Y., Ruiqi, P., Jia, D., Dezhou, S., Jianrong, W., Xiaoyun, L., Huancheng, M. (2022), Antifungal action and induction of resistance by Bacillus sp. strain YYC 155 against Colletotrichum fructicola for control of anthracnose disease in Camellia oleifera, Frontiers in Microbiology, 13, 1–15.
  22. Xiaolin, C., Miaomiao, Z., Lihua, T., Suiping, H., Tangxun, G., Qili, L. (2023), Screening and characterization of biocontrol bacteria isolated from Ageratum conyzoides against Collectotrichum fructicola causing Chinese plum (Prunus salicina Lindl.) anthracnose, Front. Microbiol., Antimicrobials, Resistance and Chemotherapy, (14).
  23. Trần Thùy Trang, Nguyễn Thị Ánh Nguyệt, Lê Thị Mai Châm, Nguyễn Tấn Đức, Phạm Nguyễn Đức Hoàng, Dương Hoa Xô (2020), Phân lập và tuyển chọn chủng vi khuẩn thuộc nhóm Bacillus subtilis có khả năng đối kháng tốt với nấm Colletotrichum scovillei gây bệnh thán thư trên ớt ở Thành phố Hồ Chí Minh, Tạp chí khoa học Đai học Mở thành phố Hồ Chí Minh, 15(1), 72–86.
  24. Li, H., Zhou, G., Liu, J. (2009), Isolation and identification of endophytic bacteria antagonistic to Camellia oleifera leaf blight base on informatics, 2009 2nd International Conference Biomedical Engineering Informatics, 3(6), 315–318.
  25. Nguyen Thi Nhu Huynh, Quach Van Cao Thi, Nguyen Trung Truc, Tran Quoc Dung (2022), Isolation of bacilluswith active active anti-fishing collections Colletotrichum spp. causes anthracnose on papaya fruit postharvest, TNU Journal of Science and Technology, 228(01), 357–363.
  26. Fang, G., Xiaoqing, L., Ruyue, D., Min, L., Xing, Q., Xiaolu, W., Wei, Z., Huoqing, H., Huiying, L., Bin, Y., Yuan, W., Tao, T. (2023), Exploring the antifungal mechanism of β-1,3-glucanase for effectively inhibiting the food contamination by Aspergillus flavus and Aspergillus fumigatus, Food Science and Technology, 187.