The Isolation and selection of potential Lactobacillus probiotic strains against Gram–negative bacteria causing diarrhea in chickens
Vol. 133 No. 3C (2024), Original Article
Vol. 133 No. 3C (2024)

The Isolation and selection of potential Lactobacillus probiotic strains against Gram–negative bacteria causing diarrhea in chickens

Original Article
https://doi.org/10.26459/hueunijard.v133i3C.7611
Published 2024-09-30
Hoang Thi Anh Phuong
Tay Nguyen University, 567 Le Duan St., Buon Ma Thuot, Dak Lak, Vietnam
Phan Vu Hai
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Le Thi Kieu Giang
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Le Minh Duc
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Nguyen Dinh Thuy Khuong
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Nguyen Xuan Hoa
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam

Keywords

probiotic
kháng kháng sinh
Lactobacillus
E. coli
Salmonella antibiotic resistance
Lactobacillus
E. coli
probiotic
Salmonella spp.

Abstract

This study aimed to isolate and select Lactobacillus strains from indigenous chicken feces, which were collected from rural areas surrounding Hue City, capable of counteracting Escherichia coli FG31-1 and Salmonella typhimurium FC13827, which cause diarrhea in chickens. Among the 67 isolated Lactobacillus spp., 7 strains were selected based on their high antibacterial activity against both pathogens. After confirming their safety (no hemolytic activity and no potential for antibiotic resistance gene transfer), 5 strains were identified using 16S rRNA sequencing to confirm their belonging to the Lactobacillus genus. The result showed that the identified strains include LA8 (L. plantarum 1582), LA3 (L. plantarum JDM1), LA18 (L. acidophilus NCFM), LA5 (L. agilis DSM 20509), and LA36 (L. agilis La3). Among them, L. plantarum 1582 was selected as the most promising strain based on its superior probiotic characteristics, such as extracellular enzyme production (amylase and protease), adhesion to intestinal mucosa (hydrophobicity, auto/co-aggregation ability), and tolerance to acidic and bile salt conditions of the chicken gastrointestinal tract. In conclusion, this finding provides a scientific basis for using Lactobacillus-based probiotics as a potential alternative to antibiotics in combating gastrointestinal pathogens in poultry farming.

https://doi.org/10.26459/hueunijard.v133i3C.7611

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