EFFECT OF CULTURE CONDITIONS ON β-1,3-GLUCANASE PRODUCTION BY Paenibacillus polymyxa M6
Vol. 133 No. 3D (2024), Original Article
Vol. 133 No. 3D (2024)

EFFECT OF CULTURE CONDITIONS ON β-1,3-GLUCANASE PRODUCTION BY Paenibacillus polymyxa M6

Original Article
https://doi.org/10.26459/hueunijard.v133i3D.7706
Published 2024-12-31
Hoang Ha My A
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
Vo Thi Ngoc Trai
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
Nguyen Bao Hung
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam

Keywords

β-1,3-glucanase
Paenibacillus polymyxa
Culture conditions
Thua Thien Hue

Abstract

In this study, we isolated and optimized the culture conditions for β-1,3-glucanase biosynthesis by a highly enzyme-producing bacterial strains isolated in Thua Thien Hue province. Among the three isolates M6, DH2, and T5, strain M6 exhibited the strongest β-1,3-glucanase production with the strongest hydrolysis zone of 19.03±0.05 mm diameter. Strain M6 was identified through nucleotide sequencing by 16S rRNA region, showing a similarity of 99.72% with Paenibacillus polymyxa HGPJ-62 in the GenBank database (NCBI). The optimized medium composition and culture conditions for strain Paenibacillus polymyxa M6 were determined as follows: the β-1,3-glucanase induction medium comprised the following components: K₂HPO₄·3H₂O 1.3 g; MgSO₄·7H₂O 1 g; KCl 0.5 g; yeast extract 0.5 g; NaNO₃ 1 g; oat flour 5 g; pH = 6, incubation temperature 40–50°C for 24 hours, shaking speed 180 rpm, and inoculum ratio 1%. The β-1,3-glucanase activity reached 1.5 U/mL. The present study highlights the β-1,3-glucanase biosynthesis by strain M6, demonstrating its suitability for development and application for prevent gummosis disease in Thanh Tra pomelo trees as well as in controlling fungal pathogens with glucan-dominant cell walls.

https://doi.org/10.26459/hueunijard.v133i3D.7706

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