Effect of extraction temperatures on in vitro antioxidant activities of polysaccharides from Ophiocordyceps sobolifera


Ophiocordyceps sobolifera
extraction temperature
antioxidant activity

How to Cite

Khoa TV, Hieu LT, Son LL, Vu HXA, Kiet TT, Thi TTV. Effect of extraction temperatures on in vitro antioxidant activities of polysaccharides from Ophiocordyceps sobolifera. HueUni-JNS [Internet]. 2019Nov.11 [cited 2020Oct.30];128(1D):17-21. Available from: http://jos.hueuni.edu.vn/index.php/HUJOS-NS/article/view/5273


The objective of this paper is to evaluate the effects of extraction temperatures on in vitro antioxidant potential of polysaccharides from the Ophiocordyceps sobolifera. The antioxidant capacity of Ophiocordyceps sobolifera polysaccharides was evaluated using 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) as a radical scavenger and the total antioxidant capacity methods. The results show that the highest antioxidant activity of the polysaccharides could be attained at the extraction temperature of 80°C. The total antioxidant capacity of olysaccharides from Ophiocordyceps sobolifera is from 0.1183 ± 0.0011 to 0.1454 ± 0.0021 mg GA/g or from 0.1105 ± 0.0004 to 0.1215 ± 0.0006 μmol AS/g. The polysaccharides have the most potent antioxidant activity with the lowest half‐maximal inhibitory concentration (IC50) value ranging from 0.97 mg/mL to 1.06 mg/mL.



  1. Wang S, Liu Y, Zhang G, Zhao S, Xu F, Geng X, Wang H. Cordysobin, a novel alkaline serine protease with HIV‐1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera. Journal of Bioscience and Bioengineering. 2012;113(1):42‐47.
  2. Chiu C, Chyau C, Chen C, Lin C, Cheng C, Mong M. Polysaccharide extract of Cordyceps sobolifera attenuates renal injury in endotoxemic rats. Food and Chemical Toxicology. 2014;69:281‐288.
  3. Long X, Yan Q, Peng L, Liu X, Luo X. Effect of Various Temperatures on Bletillae Rhizoma Polysaccharide Extraction and Physicochemical Properties. Applied Sciences. 2018;9(1):116.
  4. Qu C, Yu S, Jin H, Wang J, Luo L. The pretreatment effects on the antioxidant activity of jujube polysaccharides. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2013;114:339‐343.
  5. DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry. 1956;28(3):350‐356.
  6. Nair VD, Panneerselvam R, Gopi R. Studies on methanolic extract of Rauvolfia species from Southern Western Ghats of India – In vitro antioxidant properties, characterisation of nutrients and phytochemicals. Industrial Crops and Products. 2012;39:17‐25.
  7. Megala J, Geetha A. Free radical‐scavenging and H+, K+‐ ATPase inhibition activities of Pithecellobium dulce. Food Chemistry. 2010;121(4):1120‐1128.
  8. Jayaprakasha G, Selvi T, Sakariah K. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Research International. 2003;36(2):117‐122.
  9. WONG S, LEONG L, WILLIAMKOH J. Antioxidant activities of aqueous extracts of selected plants. Food Chemistry. 2006;99(4):775‐783.
  10. Brand‐Williams W, Cuvelier M, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT ‐ Food Science and Technology. 1995;28(1):25‐30.
  11. Lu J, Gu G, Hao L, Jin Z, Wang X. Characterization and In vitro Antioxidant Activity of a Polysaccharide from Cordyceps sobolifera. Journal of Food Processing and Preservation. 2015;40(3):447‐452.
  12. Dong C, Yao Y. In vitro evaluation of antioxidant activities of aqueous extracts from natural and cultured mycelia of Cordyceps sinensis. LWT ‐ Food Science and Technology. 2008;41(4):669‐677.
  13. Jing Y, Cui X, Chen Z, Huang L, Song L, Liu T, Lv W, Yu R. Elucidation and biological activities of a new polysaccharide from cultured Cordyceps militaris. Carbohydrate Polymers. 2014;102:288‐296.
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