Screening and analysing toxic genes from Vibrio isolates causing acute hepatopancreatic necrosis disease in white-leg shrimp at Thua Thien Hue
Vol. 129 No. 1A (2020), Hue University Journal of Science: Natural Science
Vol. 129 No. 1A (2020)

Screening and analysing toxic genes from Vibrio isolates causing acute hepatopancreatic necrosis disease in white-leg shrimp at Thua Thien Hue

Hue University Journal of Science: Natural Science
https://doi.org/10.26459/hueuni-jns.v129i2A.5621
Published 2020-03-20
Hoang Tan Quang
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
https://orcid.org/0000-0002-7275-8852
Tran Thuy Lan
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Pham Thi Diem Thi
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Le Thi Tuyet Nhan
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Le My Tieu Ngoc
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Nguyen Duy Quynh Tram
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Nguyen Thi Thu Lien
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
PDF (Vietnamese)

Keywords

AHPND
acute hepatopancreatic necrosis disease
shrimp
toxic encoding gene
Vibrio AHPND
gen độc tố
hoại tử gan tụy cấp
tôm
Vibrio

How to Cite

1.
Quảng HT, Lan TT, Thi PTD, Nhân LTT, Ngọc LMT, Trâm NDQ, Liên NTT. Screening and analysing toxic genes from Vibrio isolates causing acute hepatopancreatic necrosis disease in white-leg shrimp at Thua Thien Hue. hueuni-jns [Internet]. 2020Mar.20 [cited 2024Nov.19];129(1A):115-23. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5621
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Abstract

Acute hepatopancreatic necrosis disease (AHPND) is a disease caused by bacteria, with the death ratio up to 100% in the population of Litopenaeus vannamei and Penaeus monodon, and causes great economic losses to many shrimp‐producing countries in Asia. Previous studies have shown that not all strains of Vibrio can cause AHPND because they contain different toxin genes, such as pirAvp, pirBvb, tlh, trh, and tdh. In this study, we evaluate the presence of several toxic genes on Vibrio isolates from Thua Thien Hue province and analyze the sequence of these genes. The results show that in 14 Vibrio strains carrying pirABvp gene, the tlh and toxR genes occur in 14/14 and 7/14 strains, respectively, while none of them have the two genes of trh and tdh. Analyzing the sequence of four DNA fragments shows that these genes have high similarity (98–100%) compared with the genes announced on the Genbank. Genes pirAvp and pirBvp are less different, while tlh and toxR genes are more different. The results could be used for further studies in the production of bioproducts for the prevention and treatment of acute hepatopancreatic necrosis disease in shrimp.

https://doi.org/10.26459/hueuni-jns.v129i2A.5621
PDF (Vietnamese)

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