Development of a diagnostic SCAR marker for Vibrio vulnificus causing ulcerative disease in marine fishes in Thua Thien Hue
Vol. 129 No. 1C (2020), Original Article
Vol. 129 No. 1C (2020)

Development of a diagnostic SCAR marker for Vibrio vulnificus causing ulcerative disease in marine fishes in Thua Thien Hue

Original Article
https://doi.org/10.26459/hueuni-jns.v129i1C.5780
Published 2020-06-30
Hoang Tan Quang
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Pham Thi Diem Thi
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Tran Thuy Lan
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Pham Thi Hai Yen
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Tran Quang Khanh Van
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Nguyen Duy Quynh Tram
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
PDF (Vietnamese)

How to Cite

1.
Quảng HT, Thi PTD, Lan TT, Yến PTH, Vân TQK, Trâm NDQ. Development of a diagnostic SCAR marker for Vibrio vulnificus causing ulcerative disease in marine fishes in Thua Thien Hue. hueuni-jns [Internet]. 2020Jun.30 [cited 2024Nov.21];129(1C):99-108. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5780
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Abstract

Vibrio vulnificus is widespread on cultured fish with the ulcerative disease in the Thua Thien Hue province, Vietnam, and it is a potential human pathogen that can cause primary septicemia, wound infection, and gastroenteritis in susceptible individuals. Thus, this study is conducted to develop a molecular marker generated from random amplified polymorphic DNA (RAPD) for the rapid detection of V. vulnificus. Twenty random primers were tested by using the RAPD method to detect DNA polymorphisms among Vibrio species. The random primer OPA-09 generates a 1356-bp DNA fragment specific for V. vulnificus. This sequence was used to design a specific primer, and it was transferred to a sequence-characterized amplified region (SCAR) marker with a length of 938 bp (A9-938). The specific primers Vvul-1F/Vvul-938R amplify a unique DNA fragment in all isolates of V. vulnificus but not in the other tested Vibrio species. This marker is highly specific and can be used to identify the presence of V. vulnificus in seafood.

https://doi.org/10.26459/hueuni-jns.v129i1C.5780
PDF (Vietnamese)

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