Development of a diagnostic SCAR marker for Vibrio vulnificus causing ulcerative disease in marine fishes in Thua Thien Hue
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1.
Quảng HT, Thi PTD, Lan TT, Yến PTH, Vân TQK, Trâm NDQ. Development of a diagnostic SCAR marker for Vibrio vulnificus causing ulcerative disease in marine fishes in Thua Thien Hue. HueUni-JNS [Internet]. 2020Jun.30 [cited 2020Dec.5];129(1C):99-108. Available from: http://jos.hueuni.edu.vn/index.php/HUJOS-NS/article/view/5780

Abstract

Vibrio vulnificus is widespread on cultured fish with the ulcerative disease in the Thua Thien Hue province, Vietnam, and it is a potential human pathogen that can cause primary septicemia, wound infection, and gastroenteritis in susceptible individuals. Thus, this study is conducted to develop a molecular marker generated from random amplified polymorphic DNA (RAPD) for the rapid detection of V. vulnificus. Twenty random primers were tested by using the RAPD method to detect DNA polymorphisms among Vibrio species. The random primer OPA-09 generates a 1356-bp DNA fragment specific for V. vulnificus. This sequence was used to design a specific primer, and it was transferred to a sequence-characterized amplified region (SCAR) marker with a length of 938 bp (A9-938). The specific primers Vvul-1F/Vvul-938R amplify a unique DNA fragment in all isolates of V. vulnificus but not in the other tested Vibrio species. This marker is highly specific and can be used to identify the presence of V. vulnificus in seafood.

https://doi.org/10.26459/hueuni-jns.v129i1C.5780
PDF (Vietnamese)

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