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Abstract
Gynostemma pentaphyllum (Thunb.) Makino (Jiaogulan) has long been used as folk medicine and tea in Asia. G. pentaphyllum is a perennial creeping herb belonging to the Cucurbitaceae family. It contains saponins, flavonoids, polysaccharides, vitamins, and amino acids. In this study, the in vitro propagation capacity of this species via callus induction was investigated. The results show that suitable media for callus induction are basal MS with 1.5 mg/L NAA (naphthaleneacetic acid) (for leaf) and 0.2 mg/L 2.4-D (2,4-dichlorophenoxyacetic acid) (for petiole), and the ratio of callus induction was 100% and 97.8%, respectively. Shoots grow from callus on the MS medium with 2.0 mg/L BAP (6-Benzylaminopurine) and 0.2 mg/L NAA at a rate of 55.6%. MS medium containing 1.0 mg/L BAP has the highest shoot multiplication efficiency for apical buds (6.17 shoots/sample) while MS with 0.3 mg/L BAP has the highest efficiency for lateral buds (7.72 shoots/sample). The MS medium with 0.5 mg/L NAA is suitable for rooting at a rate of 7.22 roots/shoot.
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