Detection of the cpb gene in Clostridium perfringens isolated from chickens with necrotic enteritis in Hue City
Vol. 135 No. 3B (2026), Original Article
Vol. 135 No. 3B (2026)

Detection of the cpb gene in Clostridium perfringens isolated from chickens with necrotic enteritis in Hue City

Original Article
Published 2026-06-03
Nguyen Dinh Thuy Khuong
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Nguyen Xuan Hoa
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Huynh Van Chuong
Institute of Biotechnology, Hue University, Nguyen Dinh Tu St., Hue City
Ta Dang Y Vi
Institute of Biotechnology, Hue University, Nguyen Dinh Tu St., Hue City
Hoang Nhat Ha
Institute of Biotechnology, Hue University, Nguyen Dinh Tu St., Hue City
Nguyen Bao Hung
Institute of Biotechnology, Hue University, Nguyen Dinh Tu St., Hue City

Keywords

poultry diseases
Clostridium perfringens
broiler chicken
cpb gene
Hue City
necrotic enteritis gia cầm
Clostridium perfringens
gà
gen cpb
Thành phố Huế
viêm ruột hoại tử

Abstract

The study was conducted to isolate and identify the presence of the cpb gene in Clostridium perfringens from clinical samples collected in Hue City. A total of 200 fecal samples were collected from chickens suspected of having necrotic enteritis across four areas: Huong Thuy, Quang Dien, Phu Vang, and Huong Tra (former). Using anaerobic cultivation and traditional biochemical characterization, 116 C. perfringens strains were isolated, accounting for a rate of 58.00%. The infection rate tended to increase with farm scale, reaching the highest in large-scale farms (66.67%, p < 0.05), while no significant differences were observed between age groups (p > 0.05). All isolates exhibited typical phenotypic and biochemical characteristics of the species. Notably, through PCR technique, the study identified that 28 out of 116 isolates carried the cpb toxin gene, accounting for 24.14%. These results indicate the presence of strains harboring the cpb gene circulating locally. The study provides important baseline data for epidemiological forecasting and the development of necrotic enteritis control strategies in the region.

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