GENETIC DIVERSITY OF ANOECTOCHILUS ROXBURGHII IN HUE CITY, VIETNAM, BASED ON RAPD MARKERS
Tập 134 Số 3D (2025), Nghiên Cứu
Tập 134 Số 3D (2025)

GENETIC DIVERSITY OF ANOECTOCHILUS ROXBURGHII IN HUE CITY, VIETNAM, BASED ON RAPD MARKERS

Nghiên Cứu
https://doi.org/10.26459/hueunijard.v134i3D.7774
Đã xuất bản 2025-06-20
Nhi Thi Hoang Ho
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
Han Ngoc Ho
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
Tien Quang Duc Nguyen
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Hồng Hải TTrương
Institute of Biotechnology, Hue University, Nguyen Đinh Tu St., Hue, Vietnam
https://orcid.org/0000-0002-6591-5485
PDF (English)

Từ khóa

english Anoectochilus roxburghii
genetic diversity
jewel orchid
RAPD
Vietnam

Tóm tắt

Anoectochilus roxburghii (Wall.) Lindl, a valuable medicinal plant, is under threat due to overexploitation and adverse growing conditions. Therefore, genetic research is necessary to preserve and create hybrid varieties for breeding. In this study, we used RAPD markers to assess genetic differences among 17 A. roxburghii accessions collected in Hue City. The results showed that 17 RAPD selected primers were suitable for evaluating genetic polymorphism due to the high percentage of polymorphic bands (76.105%) and the na, ne, h, and I indices gave high values, reaching 1.762, 1.428, 0.249 and 0.373, respectively. Among these 17 primers, UBC#405, UBC#427, UBC#446, UBC#469, UBC#476 and UBC#498 were the six most optimal primers for RAPD marker development with the highest PIC, Rp, MI, and EMR indices. Additionally, the UPGMA tree was separated into three clusters, which showed the genetic grouping among accessions in the population of A. roxburghii. The similarity coefficients ranged from 0.635 to 0.930. HUIB_AR10 was most similar to HUIB_AR06 while it was most different from HUIB_AR01.

https://doi.org/10.26459/hueunijard.v134i3D.7774
PDF (English)

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