IN VITRO PROPAGATION OF Amomum sp. FROM A LUOI DISTRICT OF THUA THIEN HUE PROVINNCE

Abstract

Amomum is a precious medicinal plant, currently being developed and cultivated to produce the source of medicinal materials. This paper presents the obtained results of micropropagation of Amomum sp., originated in Hong Thuy commune, A Luoi district, Thua Thien Hue province. Shoot tips and stem bases were sterilized with HgCl₂ 0.15% in 15-18 minutes, then soaked nano silver solution 40 ppm in 12 minutes. Results indicated that highly effective sterilization occurred with HgCl₂ in 16 minutes for shoot tips (the highest rate of living explants was 40%) and in 17 minutes for stem bases (the highest rate of living explants was 42.86%). Then, shoot tips and stem bases were cultured in MS medium supplemented alone with BAP, kinetin. After 4 weeks of culture, the best regeneration buds achieved on MS medium supplemented with 2.0 mg/l BAP (1.83 shoots/stem base; 2.75 shoots/shoot tip were cut longitudinally into two pieces) or on MS medium supplemented with 3.0 mg/l kinetin (1.60 shoots/stem base; 2.0 shoots/shoot tip were cut longitudinally into two pieces). The shoot multiplication from in vitro stem base was achieved on MS medium supplemented alone with BAP, kinetin or in combination BAP with NAA. After 10 weeks of culture, the multiplication coefficent was greatest on MS medium supplemented with 1.0 mg/l BAP. The average number of shoots per explant was 9.83. In vitro shoots were transfered on MS medium supplemented with NAA or IBA for root initiation. The highest number of roots per shoot (7.17) was obtained on MS medium supplemented with 0.6 mg/l NAA. The longest roots (3.89 cm) observed in MS medium supplemented with IBA 0.4 mg/l. The plantlets were acclimatized and transplanted successfully in the coconut coir pots. The plantlet survival rate was 94.44% one month after transferring in pots and 72.22% two month after transferring in pots.