Fourier transform infrared characterization of salicylic acid treated rice plants against Xanthomonas oryzae pv. oryzae

Abstract

Salicylic acid (SA) plays an important role in induction of plant defence against a wide range of biotic and abiotic stresses through physiological, biochemical and molecular responses. The study was carried out to evaluate defence reponses of SA- induced rice plants against leaf blight (LB) disease, caused by Xanthomonas oryzae pv. oryzae (Xoo). In this study, a series of SA solutions at concentrations of 0.25, 0.50, 0.75, 1.0, 2.0 and 4.0 mM were evaluated for their potential to protect rice LB in the net house conditions. Biochemical anal-yses were characterized by means of a FTIR spectroscope. Our results showed that at inocu-lated leaves, the elicitor of SA at a concentration of 1.0 mM had highest reduction of disease severity approximately 34 % at 21 days after inoculation, compared to the control. Moreo-ver, SA application and Xoo inoculation were efficient for enhancing of carbohydrates, lipids and proteins modifications at both leaves above and below the Xoo-inoculated leaf. These results collectively suggest that the elicitor of SA strongly induced the systemic resistance in rice plants.