Isolation and DNA cloning of thermolabile hemolysin gene of Vibrio bacteria from Sciaenops ocellatus in Thua Thien Hue
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Long Đặng T, Hoàng NT, Hồng HTK, Trâm LLT, Yến PTH, Chương HV, Trang NTQ, Hiệp NV. Isolation and DNA cloning of thermolabile hemolysin gene of Vibrio bacteria from Sciaenops ocellatus in Thua Thien Hue. hueuni-jns [Internet]. 2019Oct.25 [cited 2023Sep.28];128(1E):5-14. Available from:


In this study, we isolated and identified the Vibrio parahaemolyticus 01 strain in Thua Thien Hue province causing ulcer disease in Sciaenops ocellatus. The full-length of thermolabile hemolysin (tlh) gene (1257 bp), encoding antigen thermolabile hemolysin toxin (TLH) of the Vibrio sp. was cloned and sequenced successfully. The sequence analysis of gene cloned shows a complete similarity to the Vibrio parahaemolyticus strain (Genbank: AY289609.1). Gene tlh encodes a complete polypeptide sequence of 418 amino acids and completely consistent with polypeptide chains published in Genebank (accession number: AAP41840.1). Our findings show a high potential of the PCR-based method for rapid identification and providing genetic relationship information and isolate the toxin gene from Vibrio bacteria in Thua Thien Hue province.
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  1. Subasinghe RP, Bondad-Reantaso MG, McGladdery SE. Aquaculture development, health and wealth. 2001 In: Aquaculture in the Third Millennium. Technical Proceedings of the Conference on Aquaculture in the Third Millennium ed., Subasinghe RP, Bueno P, Phillips MJ, Hough C, McGladdery SE, et al., (Eds.) Rome, Italy: NACA, Bangkok and FAO, 167-91.
  2. Chatterjee S, Haldar S. Vibrio Related Diseases in Aquaculture and Development of Rapid and Accurate Identification Methods. Journal of Marine Science: Research&Development. 2012;s1.
  3. John KR, George MR. Viruses associated with epizootic ulcerative syndrome: an update. Indian Journal of Virology. 2012;23(2):106–13.
  4. Fraser C, Hanage WP, Spratt BG. Recombination and the nature of bacterial speciation. Science. 2007;26:476-80.
  5. Egidius E. Vibriosis : pathogenicity and pathology. A review Aquaculture. 1987;67:15-28.
  6. Hendrikson RG, Zenoble RD.Vibriosis in fish : a review. Iowa State University Veterinarian. 1983;45:25-8.
  7. Shinoda S. Protein toxins produced by pathogenic vibrios. Journal of natural toxins. 1999;8:259-69.
  8. Zhang XH, Austin B. Haemolysins in Vibrio species. Journal of Applied Microbiology. 2005;98:1011-19.–2672.2005.02583.x
  9. Taniguchi H, Ohta H, Ogawa M, Mizuguchi Y. Cloning and expression of Escherichia coli of Vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes. Journal of Bacteriology. 1985;162:510-15.
  10. Taniguchi H, Hirano H, Kubomura S, Higashi K, Mizuguchi Y. Comparison of the nucleotide sequences of the genes for the thermostable direct hemolysin and the thermolabile hemolysin from Vibrio parahaemolyticus. Microbial Pathogenesis. 1986;1:425-32.
  11. Shinoda S, Matsuoka H, Tsuchie T, Miyoshi S, Yamamoto S, Taniguchi H, Mizuguchi Y. Purification and characterization of a lecithin-dependent haemolysin from Escherichia coli transformed by a Vibrio parahaemolyticus gene. Journal of general microbiology. 1991;137:2705-11.
  12. Arunagiri K, Jayashree K, Sivakumar T. Isolation and identification of Vibrios from marine food resources. International Journal of Current Microbiology and Applied Sciences. 2013;2(7):217-32.
  13. McCarthy SA, DePaola A, Cook DW, Kaysner CA, Hill WE. Evaluation of alkaline phosphatase- and digoxigenin-labelled probes for detection of the thermolabile hemolysin (tlh) gene of Vibrio parahaemolyticus. Letters in Applied Microbiology. 1999;28:66-70.
  14. Panicker G, Call DR, Krug MJ, Bej AK. Detection of Pathogenic Vibrio spp. in Shellfish by Using Multiplex PCR and DNA Microarrays. Applied and Environmental Microbiology. 2004;70(12):7336-444.–7444.2004
  15. Lụa ĐT, Khuê NV, Vân PT. Non-Vibrio parahaemolyticus gây bệnh hoại tử gan tụy cấp (AHPND) trên tôm nuôi. Tạp chí Khoa học Nông nghiệp Việt Nam. 2016;14(5):690-8.
  16. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution. 1993;10:512-26.
  17. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution. 2016;33:1870-4.
  18. Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG. Primer3--new capabilities and interfaces. Nucleic Acids Research. 2012;40(15):115.
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