Isolation and DNA cloning of thermolabile hemolysin gene of Vibrio bacteria from Sciaenops ocellatus in Thua Thien Hue
Vol. 128 No. 1E (2019), Special Issues - Conference
Vol. 128 No. 1E (2019)

Isolation and DNA cloning of thermolabile hemolysin gene of Vibrio bacteria from Sciaenops ocellatus in Thua Thien Hue

Special Issues - Conference
https://doi.org/10.26459/hueuni-jns.v128i1E.5373
Published 2019-10-25
Đặng Thanh Long
Viện Công nghệ sinh học, Đại học Huế
Nguyễn Thái Hoàng
Trường Trung học phổ thông Lê Lợi, Pleiku, Gia Lai
Hoàng Thị Kim Hồng
Trường Đại học Khoa học, Đại học Huế
Lê Lý Thùy Trâm
Trường Đại học Bách Khoa, Đại học Đà Nẵng
Phạm Thị Hải Yến
Trường Đại học Nông Lâm, Đại học Huế
Huỳnh Văn Chương
Viện Công nghệ sinh học, Đại học Huế
Nguyễn Thị Quỳnh Trang
Trường Đại học Sư phạm, Đại học Huế
Nguyễn Văn Hiệp
Viện Công nghệ sinh học, Đại học Huế
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How to Cite

1.
Long Đặng T, Hoàng NT, Hồng HTK, Trâm LLT, Yến PTH, Chương HV, Trang NTQ, Hiệp NV. Isolation and DNA cloning of thermolabile hemolysin gene of Vibrio bacteria from Sciaenops ocellatus in Thua Thien Hue. hueuni-jns [Internet]. 2019Oct.25 [cited 2024Dec.21];128(1E):5-14. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5373
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Abstract

In this study, we isolated and identified the Vibrio parahaemolyticus 01 strain in Thua Thien Hue province causing ulcer disease in Sciaenops ocellatus. The full-length of thermolabile hemolysin (tlh) gene (1257 bp), encoding antigen thermolabile hemolysin toxin (TLH) of the Vibrio sp. was cloned and sequenced successfully. The sequence analysis of gene cloned shows a complete similarity to the Vibrio parahaemolyticus strain (Genbank: AY289609.1). Gene tlh encodes a complete polypeptide sequence of 418 amino acids and completely consistent with polypeptide chains published in Genebank (accession number: AAP41840.1). Our findings show a high potential of the PCR-based method for rapid identification and providing genetic relationship information and isolate the toxin gene from Vibrio bacteria in Thua Thien Hue province.

https://doi.org/10.26459/hueuni-jns.v128i1E.5373
PDF (Vietnamese)

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