Keywords
DPPH
microwave
phenolic
response surface methodology betacyanin
bề mặt đáp ứng
DPPH
phenol
vi sóng
How to Cite
Abstract
Betacyanin has been widely used as natural coloring agents and dyes in food and textile industries to replace conventional toxic chemical pigments. In this study, the extraction of betacyanin from red dragon fruit peels was optimized using response surface methodology. Betacyanin was determined as the response to three independent variables, namely the ratio of extraction solvent to peels, microwave-assisted treatment time, and pH. The effect of acid ascorbic concentration during the storage of betacyanin extract, the total phenolic content, and DPPH radical scavenging activity were also investigated. The optimum extraction parameters are as follows: the ratio of extraction solvent to peels is 53% (v/w); extraction time is 91 s; pH is 5.3, with the betacyanin content being 72.13 mg/100 g peels. The betacyanin extract supplemented with 0.5% (w/v) ascorbic acid has the longest storage duration. Total phenolic concentration and DPPH radical scavenging activity (IC50) are 41.41 mg GAE/100 mL and 48.02 % (v/v), respectively.
References
- Celli GB, Brooks MS. Impact of extraction and processing conditions on betalains and comparison of properties with anthocyanins — A current review. Food Research International. 2017;100:501-509.
- Moßhammer M, Stintzing F, Carle R. Colour studies on fruit juice blends from Opuntia and Hylocereus cacti and betalain-containing model solutions derived therefrom. Food Research International. 2005;38(8-9):975-981.
- Linh ĐTM, Mai NTQ, Duyên HT, Nghi TH. Nghiên cứu quá trình tạo bột màu betacyanin thu nhận từ vỏ quả thanh long (Hylocereus undatus). Tạp chí Khoa học Công nghệ và Thực phẩm. 2018;17(1):21-31.
- Cảnh N. Quy hoạch thực nghiệm, Nhà xuất bản Đại học Quốc gia Thành phố Hồ Chí Minh; 2016.
- Sharma S, Hullatti KK, Kumar S, Brijesh KK. Comparative antioxidant activity of Cuscuta reflexa and Cassytha filiformis. Journal of Pharmacy Research. 2012;1:441-443.
- Ramli NS, Ismail P, Rahmat A. Influence of Conventional and Ultrasonic-Assisted Extraction on Phenolic Contents, Betacyanin Contents, and Antioxidant Capacity of Red Dragon Fruit (Hylocereus polyrhizus). The Scientific World Journal. 2014;2014:1-7.
- Woo K, Ngou F, Ngo L, Soong W, Tang P. Stability of Betalain Pigment from Red Dragon Fruit (Hylocereus polyrhizus). American Journal of Food Technology. 2011;6(2):140-148.
- Wong Y, Siow L. Effects of heat, pH, antioxidant, agitation and light on betacyanin stability using red-fleshed dragon fruit (Hylocereus polyrhizus) juice and concentrate as models. Journal of Food Science and Technology. 2014;52(5):3086-3092.
- Bilyk A, Howard M. Reversibility of thermal degradation of betacyanines under the influence of isoascorbic acid. Journal of Agricultural and Food Chemistry. 1982;30(5):906-908.
- Quế PTT, Thủy NTT, Ngọc TTA, Nghĩa LD. Ảnh hưởng của điều kiện chế biến và bảo quản đến sự ổn định màu betacyanin trong nước ép thịt quả thanh long ruột đỏ (Hylocereus polyrhizus). Tạp chı́ Khoa học Trường Đại học Cần Thơ. 2017;51:16-23.
- Wu L, Hsu H, Chen Y, Chiu C, Lin Y, Ho JA. Antioxidant and antiproliferative activities of red pitaya. Food Chemistry. 2006 03;95(2):319-327.
- Naczk M, Shahidi F. Extraction and analysis of phenolics in food. Journal of Chromatography A. 2004 Oct 29;1054(1-2):95-111.
- Pichayajittipong P, Thaiudom S. Optimum Condition of Beta-Cyanin Colorant Production from Red Dragon Fruit (Hylocercus polyrhizus) Peels using Response Surface Methodology. Chiang Mai University Journal of Natural Sciences. 2014;13(1):483-496.
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