In vitro propagation of Dendrobium anosmum mutation
Vol. 131 No. 1A (2022), Original Article
Vol. 131 No. 1A (2022)

In vitro propagation of Dendrobium anosmum mutation

Original Article
https://doi.org/10.26459/hueunijns.v131i1A.6556
Published 2022-03-31
Le Vinh Thien
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue
Truong Thi Ly Na
Quang Tri Technical College, 179 Ly Thuong Kiet st., Quang Tri, Vietnam
Nguyen Thi Bich Thu
Quang Tri Technical College, 179 Ly Thuong Kiet St., Quang Tri, Vietnam
Nguyen Thi Diem
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Nguyen Thi Oanh
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Nguyen Thi Kim Cuc*
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue
PDF (Vietnamese)

Keywords

Giả hạc đột biến
gieo hạt
in vitro Dendrobium anosmum
in vitro
mutant orchids
seedings

How to Cite

1.
Lê VT, Trương TLN, Nguyễn TBT, Nguyen TD, Nguyễn TO, Nguyễn TKC. In vitro propagation of Dendrobium anosmum mutation. hueuni-jns [Internet]. 2022Mar.31 [cited 2024Nov.21];131(1A):5-15. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/6556
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Abstract

Currently, mutant orchids are very interested in orchid lovers who want to collect, but the price of mutant orchids is costly leading to many orchid lovers are unable to own it. This study aims to create mutant orchid plantlets with high quantity and low cost via in vitro propagation method. From the self-pollinated six months old seedpods, we have successfully propagated the orchid mutant seedlings. The results showed that the suitable medium for mutant orchid seed germination and creating protocorm was the basic MS medium supplemented with 0.5 mg/L BA with a rate of up to 93%. The medium for shoot formation and shoot development from protocorm was the best on MS medium added with 1.0 mg/L BA and 0.5 mg/L IBA with a rate of 88%. The rooting induction was 99.06% and the root length reached 5.34 cm with an average of 8.53 roots/shoot when shoots cultured on MS medium supplemented with 1.0 mg/L NAA. The survival rate of in vitro Dendrobium anosmum mutant seedlings after 60 days in the nursery stage was 84.9% on coir and rice husks substrates (1:1).

https://doi.org/10.26459/hueunijns.v131i1A.6556
PDF (Vietnamese)

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