trnL-trnF gene region analysis of natural Curuligio orchioides collected in Thua Thien Hue
Vol. 132 No. 1A (2023), Original Article
Vol. 132 No. 1A (2023)

trnL-trnF gene region analysis of natural Curuligio orchioides collected in Thua Thien Hue

Original Article
https://doi.org/10.26459/hueunijns.v132i1A.6958
Published 2023-03-31
Bui Le Thanh Nhan*
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
Truong Thi Bich Phuong
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Hoang Tan Quang
Institute of Biotechnology, Hue University, Hue, Vietnam
PDF (Vietnamese)

Keywords

Sâm cau
đa dạng di truyền
trnF
trnL Curculigo orchioides
genetic diversity

How to Cite

1.
Bùi LTN, Trương TBP, Hoàng TQ. trnL-trnF gene region analysis of natural Curuligio orchioides collected in Thua Thien Hue. hueuni-jns [Internet]. 2023Mar.31 [cited 2024Jun.26];132(1A):75-82. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/6958
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Abstract

Curculigo orchioides, a member of the genus Curculigo and the family Hypoxidaceae, is a herbal plant and has been used as traditional medicine. Currently, there is high demand for cultivating this plant. However, there has not been sufficient research into the genetic diversity to assist the authentication of the material, for example, the genetic research based on the DNA indicators of Curculigo. In this study, the gene trnL-trnF region of 15 Curculigo orchioides germplasms collected from three locations in the province were sequenced and analyzed for identification and evolution. The results show that the sequenced fragment of the gene trnL-trnF from the surveyed samples has 99% similarity and 100% coverage compared with the NC 053892 sequence of Curculigo orchioides. We identified five haplotypes of C. orchioides in the province with a haplotype diversity coefficient (Hd) of 0.705 ± 0.088. The genetic distances ranged from 0 to 0.49% among the populations. The Curculigo orchioides population in Thua Thien Hue has evolved towards balanced, neutral, and random selection because of the lack of rare alleles.

https://doi.org/10.26459/hueunijns.v132i1A.6958
PDF (Vietnamese)

References

  1. Bân NT. Sách đỏ Việt Nam. Phần II: Thực vật. Hà Nội: NXB Khoa Học Tự Nhiên và Công Nghệ; 2007. 691 p.
  2. Viện Dược liệu. Cây thuốc và động vật làm thuốc Việt Nam Hà Nội: Nxb Khoa học và Kỹ thuật; 2006.
  3. Murali VP, Kuttan G. Curculigo orchioides Gaertn Effectively Ameliorates the Uro- and Nephrotoxicities Induced by Cyclophosphamide Administration in Experimental Animals. Integrative cancer therapies. 2016;15(2):205-15.
  4. Lợi ĐT. Những cây thuốc và vị thuốc Việt Nam. 14, editor. Hà Nội: Nxb Y học; 2004. 910 p.
  5. Srivastava RaDK. Ethnobotanical Studies on Curculigo orchioides from Sonebhadra District of Uttar Pradesh Indian Journal of Science Research. 2014;5(2):123-4.
  6. Madhavan V, Joshi R, Murali A, Yoganarasimhan SN. Antidiabetic Activity of Curculigo orchioides Root Tuber. Pharmaceutical Biology. 2007;45(1):18-21.
  7. Chauhan NS, Sharma V, Thakur M, Dixit VK. Curculigo orchioides: the black gold with numerous health benefits. Journal of Chinese integrative medicine. 2010;8(7):613-23.
  8. Chukwuma S. Ezeonu, Chigozie M. Ejikeme. Qualitative and Quantitative Determination of Phytochemical Contents of Indigenous Nigerian Softwoods. New Journal of Science. 2016;2016:1-9.
  9. Anandakirouchenane E, Chandiran IS, Kadalmani B. An Investigation on Preliminary Phytochemical and Safety Profiles of Methanolic Root Extract of Curculigo orchioides. Journal of Pharmacy Research. 2013;7(8):692-6.
  10. Tan S, Xu J, Lai A, Cui R, Bai R, Li S, et al. Curculigoside exerts significant anti‑arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF‑kappaB signaling pathway. Mol Med Rep. 2019;19(3):2057-64.
  11. Chopra R, Nayar S, Chopra I. Glossary of Indian Medicinal Plants. The Quarterly Review of Biology. 1956;33(2):330ps.
  12. Pandit P, Singh A, Bafna A, Kadam P, Patil M. Evaluation of Antiasthmatic Activity of Curculigo orchioides Gaertn. Rhizomes. Indian Journal of Pharmaceutical Sciences. 2008;70(4):440-4.
  13. Nagesh KS, Shanthamma C. Antibacterial Activity of Curculigo orchioides Rhizome Extract on Pathogenic Bacteria. African Journal of Microbiology Research. 2009;3(1):005-9.
  14. Asif M. A Review on Phytochemical and Ethnopharmacological Activities of Curculigo orchioides. Mahidol University Journal of Pharmaceutical Sciences. 2012;39(3-4):1-10.
  15. Thakur M, Chauhan NS, Sharma V, Dixit VK, Bhargava S. Effect of Curculigo orchioides on Hyperglycemia-induced Oligospermia and Sexual Dysfunction in Male Rats. International journal of impotence research. 2012;24(1):31-7.
  16. Irshad S, Singh J, Jain SP, Khanuja SPS. Curculigo orchioides Gaertn. (Kali Musali) An endangered medical plant of commercial value. National Product Radiance. 2006;5(5):369-72.
  17. Brintha S, Rajesh S, Renuka R, Santhanakrishnan VP, Gnanam R. Phytochemical Analysis and Bioactivity Prediction of Compounds in Methanolic Extracts of Curculigo orchioides Gaertn. Journal of Pharmacognosy and Phytochemistry 2017;6(4):192-7.
  18. Dutta S, Ghosh R. DNA Barcoding: The Conspicuous Scan. In: Saranraj P, editor. Compendium of "Research Insights of Life Science Students (Rilss)". India: JPS Scientific Publications; 2022. p.1309-13.
  19. Emerson BC, Cicconardi F, Fanciulli PP, Shaw PJ. Phylogeny, Phylogeography, Phylobetadiversity and the Molecular Analysis of Biological Communities. Philosophical Transactions of the Royal Society B: Biological Sciences. 2011;366(1576) :2391-402.
  20. Guo M, Yuan C, Tao L, Cai Y, Zhang W. Life Barcoded by DNA Barcodes. Conservation Genetics Resources. 2022:1-15.
  21. Hebert PD, Penton EH, Burns JM, Janzen DH, Hallwachs W. Ten Species in One: DNA Barcoding Reveals Crypticspecies in the Neotropical Skipper Butterflyastraptes Fulgerator. PNAS. 2004;101(41): 14812-7.
  22. Group1 CPW, Hollingsworth PM, Forrest LL, Spouge JL, Hajibabaei M, Ratnasingham S, et al. A DNA barcode for land plants. PNAS. 2009;106(31):12794-7.
  23. Yao H, Song J, Liu C, Luo K, Han J, Li Y, et al. Use of ITS2 Region as the Universal DNA Barcode for Plants and Animals. PLoS One. 2010;5(10):e13102.
  24. Taberlet P, Gielly L, Pautou G, Bouvet J. Universal Primers for Amplification of Three Non-coding Regions of Chloroplast DNA. Plant Mol Biol. 1991;17(5):1105-9.
  25. Yulita KS. Secondary Structures of Chloroplast trnL Intron in Dipterocarpaceae and its Implication for the Phylogenetic Reconstruction. HAYATI Journal of Biosciences. 2013;20(1):31-9.
  26. Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, Guirao-Rico S, Librado P, Ramos-Onsins SE, et al. DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets. Molecular Biology and Evolution. 2017;34(12):3299-302.
  27. Nidhi Soni, V. K. Lal, Shikha Agrawal, Verma H. Golden eye grass - a magical remedy by nature. International Journal of Pharmatical Sciences and Research. 2012;3(8).
  28. Frankham R, Ballou JD, Briscoe DA. Introduction to Conservation Genetics: Cambridge: Cambridge University Press; 2002.
  29. Hà TTT, Hương NT, Thảo NP, Hằng TNA. Mức độ đa dạng di truyền của một số quần đàn cá tra sử dụng chỉ thị phân tử cytochrome b. Tạp chí khoa học, Trường Đại học Vinh. 2017;46(4A):21-31.
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