Detection of hypoglycemic drugs adulterated in herbal products with high-performance liquid chromatography
Vol. 132 No. 1A (2023), Original Article
Vol. 132 No. 1A (2023)

Detection of hypoglycemic drugs adulterated in herbal products with high-performance liquid chromatography

Original Article
https://doi.org/10.26459/hueunijns.v132i1A.6975
Published 2023-03-31
Le Thi Bao Tram
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
Ta Nguyen Khanh Ha
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
Hoang Thi Minh Nguyet
Hue Central Hospital, 16 Le Loi St., Hue, Vietnam
Pham Thi Thanh Ha
Hanoi University of Pharmacy, 13–15 Le Thanh Ton St., Hanoi, Vietnam
Nguyen Thi Kieu Anh
Hanoi University of Pharmacy, 13–15 Le Thanh Ton St., Hanoi, Vietnam
Nguyen Hai Phong
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Huynh Van Chung
Buon Ma Thuot Medical University, 298 Ha Huy Tap St., Buon Ma Thuot, Daklak, Vietnam
Dao Thi Cam Minh*
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
PDF (Vietnamese)

Keywords

hypoglycemic drug
herbal product
high-performance liquid chromatography thuốc hạ glucose máu
chế phẩm dược liệu
sắc ký lỏng hiệu năng cao

How to Cite

1.
Lê TBT, Tạ NKH, Hoàng TMN, Phạm TTH, Nguyễn TKA, Nguyễn HP, Huỳnh VC, Đào TCM. Detection of hypoglycemic drugs adulterated in herbal products with high-performance liquid chromatography. hueuni-jns [Internet]. 2023Mar.31 [cited 2024Jul.3];132(1A):65-73. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/6975
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Abstract

Circular No. 10/2021/TT-BYT stipulated that several hypoglycemic drugs, such as metformin hydrochloride, are prohibited from adulterating medicinal herbal preparations. Therefore, this study aims to develop a procedure to detect five hypoglycemic drugs, including metformin hydrochloride, glibenclamide, gliclazide, glimepiride, and glipizide, adulterated in herbal preparations by using the high-performance liquid chromatography (HPLC) method. The chromatographic program includes the methanol:phosphate buffer pH 3 mobile phase 70/30 (v/v), chromatographic column C18 (250 × 4.6 mm, 5 μm), and analysis time of 25 minutes. The procedure has high specificity, accuracy and precision, according to AOAC. We detected six herbal products reacting positively with glibenclamide and one with both metformin hydrochloride and glibenclamide.

https://doi.org/10.26459/hueunijns.v132i1A.6975
PDF (Vietnamese)

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