Nitrite metabolism of several bacterial strains isolated from abattoir and swine wastewater after biogas treatment


nitrite metabolism
swine wastewater
abattoir wastewater nitrit, vi khuẩn chuyển hóa nitrit, nước thải chăn nuôi lợn, nước thải lò mổ

How to Cite

Dinh TTH, Nguyen TV, Phan DH, Tran HD, Nguyen DGC, Nguyen HD. Nitrite metabolism of several bacterial strains isolated from abattoir and swine wastewater after biogas treatment. hueuni-jns [Internet]. 2022Dec.31 [cited 2023Sep.28];131(1D):105-14. Available from:


In nitrogen treatment with biological methods, nitrite metabolism is an intermediate process that facilitates other processes involving different bacteria strains. In this study, we isolated two nitrite-oxidising bacteria strains from abattoir wastewater and wastewater from biogas tanks of an industrial pig farm in Ha Tinh province. The bacteria strains grow, develop, and metabolise nitrite at pH 6–8 and 30–37 °C. The samples with the nitrite concentration up to 750 mg·L–1 were oxidised within four days of incubation, and the nitrite metabolism rate was proportional to the concentration of nitrite tested. Under severe conditions (salinity up to 3% NaCl, a low dissolved oxygen level of 0.1 mg·L–1), the two isolated bacterial strains exhibited their effective growth and nitrite metabolism capacity. The results enrich the database of nitrite-oxidising bacteria and are prospective in wastewater treatment.


  1. Focht DD, Chang AC. Nitrification and denitrification processes related to waste water treatment. Advances in applied microbiology. 1975; 19:153-86.
  2. Lee CG, Fletcher TD, Sun G. Nitrogen removal in constructed wetland systems. Engineering in life sciences. 2009;9(1):11-22.
  3. Prakasam TBS, Loehr RC. Microbial nitrification and denitrification in concentrated wastes. Water Research. 1972;6(7):859-69.
  4. Bock E, Koops HP, Ahlers B, Harms H. Oxidation of inorganic nitrogen compounds as energy source. The Prokaryotes. 1992;(Ed. 2):414-30.
  5. Arnold JS, Derek SH. Acetate Assimilation by Nitrobacter agilis in Relation to Its "Obligate Autotrophy". Journal of Bacteriology. 1968;95(3): 844-55.
  6. Prosser JI. Nitrification. Oxford Washington: IRL Press; 1986. 217 p.
  7. Sorokin D, Tourova Tj, Schmid MC, Wagner M, Koops H-P, Kuenen GJ, et al. Isolation and properties of obligately chemolithoautotrophic and extremely alkali-tolerant ammonia-oxidizing bacteria from Mongolian soda lakes. Archives of Microbiology. 2001;176(3):170-7.
  8. Gould GW, Less H. The isolation and culture of the nitrifying organisms. Canadian Journal of Microbiology. 1960;6:299-307.
  9. Hankinson TR, Schmidt EL. An acidophilic and a neutrophilic nitrobacter strain isolated from the numerically predominant nitrite-oxidizing population of an Acid forest soil. Appl Environ Microbiol. 1988;54(6):1536-40.
  10. Josserand A, Cleyet-Marel JC. Isolation from Soils of Nitrobacter and Evidence for Novel Serotypes Using Immunofluorescence. Microbial Ecology. 1979;5:197-205.
  11. Winogradsky S. Recherches sur les organismes de la nitrification. Ann Inst Pasteur. 1890;4:213-331.
  12. Ehrich S, Behrens D, Lebedeva E, Ludwig W, Bock E. A new obligately chemolithoautotrophic, nitrite-oxidizing bacterium, Nitrospira moscoviensis sp. nov. and its phylogenetic relationship. Arch Microbiol. 1995;164(1):16-23.
  13. Lebedeva EV, Off S, Zumbrägel S, Kruse M, Shagzhina A, Lücker S, et al. Isolation and characterization of a moderately thermophilic nitrite-oxidizing bacterium from a geothermal spring. FEMS Microbiology Ecology. 2011;75(2): 195-204.
  14. Spieck E, Hartwig C, McCormack I, Maixner F, Wagner M, Lipski A, et al. Selective enrichment and molecular characterization of a previously uncultured Nitrospira-like bacterium from activated sludge. Environmental Microbiology. 2006;8(3):405-15.
  15. Norisuke U, Hirotsugu F, Yoshiteru A, Satoshi T. Isolation of Nitrospira belonging to Sublineage II from a Wastewater Treatment Plant. Microbes Environ. 2013;28(3):346–53.
  16. Hirotsugu F, Asami K, Norisuke U, Kengo M, Satoshi T. Selective isolation of ammonia-oxidizing bacteria from autotrophic nitrifying granules by applying cell-sorting and sub-culturing of microcolonies. Frontiers in microbiology. 2015;6: 1159.
  17. Fujitani H, Aoi Y, Tsuneda S. Selective Enrichment of Two Different Types of Nitrospira-like Nitrite-oxidizing Bacteria from a Wastewater Treatment Plant. Microbes Environ. 2013;28(2):236-43.
  18. Watson SW, Waterbury JB. Characteristics of Two Marine Nitrite Oxidizing Bacteria, Nitrospina gracilis nov. ten. nov. sp. and Nitrococcus mobilis nov. ten. nov. sp. Archiv für Mikrobiologie. 1971;77(3):203-30.
  19. Ma S, Zhang D, Zhang W, Wang Y. Ammonia stimulates growth and nitrite-oxidizing activity of Nitrobacter winogradskyi. Biotechnology and Biotechnological Equipment. 2014;28(1):27-32.
  20. Van Loosdrecht MCM, Jetten MSM. Microbiological conversion in nitrogen removal. Water Science and Technology. 1998;38(1):1-7.
  21. Barnes D, Bliss PJ. Biological Control of Nitrogen in Wastewater Treatment. Cambridge: Cambridge University Press; 1983.
  22. Zhao B, He YL, Hughes J, Zhang XF. Heterotrophic nitrogen removal by a newly isolated Acinetobacter calcoaceticus HNR. Bioresource Technology. 2010; 101(14):5194-200.
  23. Zhao B, An Q, He YL, Guo JS. N2O and N2 production during heterotrophic nitrification by Alcaligenes faecalis strain NR. Bioresource technology. 2012;116:379-85.
  24. Ren YX, Yang L, Liang X. he characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, Acinetobacter junii YB. Bioresource Technology. 2014;171:1-9.
  25. Fei YT, Liu DM, Luo TH, Chen G, Wu H, Li L, et al. Molecular characterization of Lactobacillus plantarum DMDL 9010, a strain with efficient nitrite degradation capacity. PLoS One. 2014; 9(11): e113792.
  26. Wu YY, Liu FJ, Li LH, Yang XQ, Deng JC, Chen SJ. Isolation and identification of nitrite-degrading lactic acid bacteria from salted fish. In Advanced materials research 2012;393:828-34.
  27. Oh CK, Oh MC, Kim SH. The depletion of sodium nitrite by lactic acid bacteria isolated from kimchi. Journal of Medicinal Food. 2014;7(1):38-44.
  28. Liu DM, Wang P, Zhang XY, Xu XL, Wu H, Li L. Characterization of nitrite degradation by Lactobacillus casei subsp. rhamnosus LCR 6013. PLoS One. 2014;9(4):e93308.
  29. 29. Zeng X, Pan Q, Guo Y, Wu Z, Sun Y, Dang Y, et al. Potential mechanism of nitrite degradation by Lactobacillus fermentum RC4 based on proteomic analysis. Journal of Proteomics. 2019;194:70-8.
  30. Esmaeilzadeh P, Darvishi S, Ebrahimi K, Mirahmadi F, Vaziri M. Consideration of lactic acid bacteria ability to reduce nitrite concentration in standard de Man Rogosa and Sharpe (MRS) broth-sodium nitrite medium during fermentation period. World Applied Sciences Journal. 2012;18(3) :430-5.
  31. Esmaeilzadeh P, Darvishi S, Assadi MM, Mirahmadi F. Effect of Lactobacillus plantarum and Lactobacillus fermentum on nitrite concentration and bacterial load in fermented sausage during fermentation. World Applied Sciences Journal. 2012;18(4):493-501.
  32. Shi J, Xia C, Tian Q, Zeng X, Wu Z, Guo Y, et al. Untargeted metabolomics based on LC–MS to elucidate the mechanism underlying nitrite degradation by Limosilactobacillus fermentum RC4. LWT. 2022;163:113414.
  33. Xia C, Tian Q, Kong L, Sun X, Shi J, Zeng X, et al. Metabolomics Analysis for Nitrite Degradation by the Metabolites of Limosilactobacillus fermentum RC4. Foods. 2022;11(7):1009.
  34. Zhou M, Ye H, Zhao X. Isolation and characterization of a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri KTB for bioremediation of wastewater. Biotechnology and Bioprocess Engineering. 2014; 19(2):231-8.
  35. Diep CN, Cam PM, Vung NH, Lai TT, My NTX. Isolation of Pseudomonas stutzeri in wastewater of catfish fish-ponds in the Mekong Delta and its application for wastewater treatment. Bioresource technology. 2009;100(16):3787-91.
  36. Zhang J, Wu P, Hao B, Yu Z. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresource technology. 2011;102(21):9866-9.
  37. Roned M. Atlas Winogradsky's Medium, Modified, Handbook of Media for Enviromental Microbiology. Boca Raton: CRC Press; 1995.
  38. Satheesh N, Prasad NBL. Optimization of Parameters for Fermentative Production of Virgin Coconut Oil by Lactobacillus fermentum NDRI 141. Journal of Food Science and Engineering. 2012;2(1): 44.
  39. Lalucat J, Bennasar A, Bosch R, García-Valdés E, Palleroni NJ. Biology of Pseudomonas stutzeri. Microbiology and molecular biology reviews. 2006; 70(2):510-47.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2023 Array