Identification and characteristics of MYB4 transcription factor related to regulation of abiotic stress tolerance in peanut
Vol. 133 No. 1B (2024), Original Article
Vol. 133 No. 1B (2024)

Identification and characteristics of MYB4 transcription factor related to regulation of abiotic stress tolerance in peanut

Original Article
https://doi.org/10.26459/hueunijns.v133i1B.7182
Published 2024-06-27
Hoang Kha
Institute of Bioactive Compounds, University of Sciences, Hue University, Hue, Vietnam
Nguyen Quang Duc Tien*
Institute of Bioactive Compounds, University of Sciences, Hue University, Hue, Vietnam
PDF

Keywords

MYB4
peanut
abiotic stress
PCR cloning

How to Cite

1.
Kha H, Nguyen QDT. Identification and characteristics of MYB4 transcription factor related to regulation of abiotic stress tolerance in peanut. hueuni-jns [Internet]. 2024Jun.27 [cited 2024Dec.22];133(1B):5-11. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7182
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Peanut (Arachis hypogaea L.), an economically valuable crop, provides protein and oil for human and animal consumption. The transcription factor MYB4 has been identified as a potential drought tolerance gene in peanut. This study aimed to isolate and characterize the MYB4 gene in the L14 peanut cultivar. The isolated AhL14_MYB4 gene was found to be 1.1 kb long, with a 663 bp coding sequence containing 3 exons and 2 introns. In silico analysis showed that AhL14_MYB4 possesses a nuclear localization signal and two DNA-binding domains characteristic of transcription factors. The findings revealed key molecular features of AhL14_MYB4 and provided insights into improving drought resistance in peanut varieties. Further research on AhL14_MYB4 may aid efforts to enhance drought tolerance in local peanut cultivars through molecular breeding or genetic engineering. Overall, this finding about preliminary characterization of the peanut MYB4 gene lays the groundwork for potential genetic improvements to this economically important crop.

https://doi.org/10.26459/hueunijns.v133i1B.7182
PDF

References

  1. Varshney RK, Pandey MK, Janila P, Nigam SN, Sudini H, Gowda MVC, et al. Marker-assisted introgression of a QTL region to improve rust resistance in three elite and popular varieties of peanut (Arachis hypogaea L.). Theoretical and Applied Genetics. 2014;127(8):1771-81.
  2. Li C, Yan C, Sun Q, Wang J, Yuan C, Mou Y, et al. The bHLH transcription factor AhbHLH112 improves the drought tolerance of peanut. BMC Plant Biology. 2021;21(1):540.
  3. Jiang C, Li X, Zou J, Ren J, Jin C, Zhang H, et al. Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties. BMC plant biology. 2021;21(1):64.
  4. Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis. Trends in plant science. 2010;15(10):573-81.
  5. Vannini C, Locatelli F, Bracale M, Magnani E, Marsoni M, Osnato M, et al. Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants. The Plant Journal. 2004;37(1):115-127.
  6. Aydin G, Yucel M, Chan MT, Oktem HA. Evaluation of abiotic stress tolerance and physiological characteristics of potato (Solanum tuberosum L. cv. Kennebec) that heterologously expresses the rice Osmyb4 gene. Plant Biotechnology Reports. 2014;8(3):295-304.
  7. Wu R, Wang Y, Wu T, Xu X, Han Z. MdMYB4, an R2R3-type MYB transcription factor, plays a crucial role in cold and salt stress in apple calli. Journal of the American Society for Horticultural Science. 2017;142(3):209-216.
  8. Wang N, Qu C, Wang Y, Xu H, Jiang S, Fang H, et al. MdMYB4 enhances apple callus salt tolerance by increasing MdNHX1 expression levels. Plant Cell, Tissue and Organ Culture (PCTOC). 2017;131(2):283-93.
  9. Yao C, Li X, Li Y, Yang G, Liu W, Shao B, et al. Overexpression of a Malus baccata MYB transcription factor gene MbMYB4 increases cold and drought tolerance in Arabidopsis thaliana. International Journal of Molecular Sciences. 2022;23(3):1794.
  10. Agarwal P, Mitra M, Banerjee S, Roy S. MYB4 transcription factor, a member of R2R3-subfamily of MYB domain protein, regulates cadmium tolerance via enhanced protection against oxidative damage and increases expression of PCS1 and MT1C in Arabidopsis. Plant Science. 2020;297:110501.
  11. Wang X-C, Wu J, Guan M-L, Zhao C-H, Geng P, Zhao Q. Arabidopsis MYB4 plays dual roles in flavonoid biosynthesis. The Plant Journal. 2020;101(3):637-52.
  12. Gonçalves S. MYB transcription factors for enhanced drought tolerance in plants. Water Stress and Crop Plants2016. p. 194-205.
  13. Liao W, Yang Y, Li Y, Wang G, Peng M. Genome-wide identification of cassava R2R3 MYB family genes related to abscission zone separation after environmental-stress-induced abscission. Scientific reports. 2016;6(1):32006.
  14. Lian W, Sun T, Meng X, Sun R, Hui F, Jiang Y, Zhao Y, et al. Overexpression of the Panax ginseng MYB4 gene enhances stress tolerance in transgenic Arabidopsis thaliana. Biol Plant. 2021;65(1):27-38.
  15. Tang M, Liu L, Hu X, Zheng H, Wang Z, Liu Y, et al. Genome-wide characterization of R2R3-MYB gene family in Santalum album and their expression analysis under cold stress. Frontiers in Plant Science. 2023;14.
  16. Zhang L, Wang Y, Sun M, Wang J, Kawabata S, Li Y. BrMYB4, a suppressor of genes for phenylpropanoid and anthocyanin biosynthesis, is down-regulated by UV-B but not by pigment-inducing sunlight in Turnip cv. Tsuda. Plant and Cell Physiology. 2014;55(12):2092-2101.
  17. Mitra M, Agarwal P, Roy S. The N-terminal MYB domains affect the stability and folding aspects of Arabidopsis thaliana MYB4 transcription factor under thermal stress. Protoplasma. 2021;258(3):633-50.
  18. Wang S, Shi M, Zhang Y, Xie X, Sun P, Fang C, et al. FvMYB24, a strawberry R2R3-MYB transcription factor, improved salt stress tolerance in transgenic Arabidopsis. Biochemical and Biophysical Research Communications. 2021;569: 93-99.
  19. Manna M, Thakur T, Chirom O, Mandlik R, Deshmukh R, Salvi P. Transcription factors as key molecular target to strengthen the drought stress tolerance in plants. Physiologia Plantarum. 2021;172(2):847-868.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2024 Array