Molecular detection methods for Pasteurella multocida and Mannheimia haemolytica

Abstract

P. multocida and M. haemolytica are the main bacteria causing pasteurellosis in household animals, leading to extensive economic losses for the livestock industry. Different traditional methods have been used since the 1980s to detect P. multocida and M. haemolytica infection. Although these methods help diagnose pasteurellosis in the early stages, they are limited in sensitivity and specificity. Therefore, modern diagnostic methods that rely on bacterial genomics to improve sensitivity, specificity, and stability have developed. This report is based on our team’s results and published papers related to diagnosis, typing, and polymorphism of pasteurellosis via PCR, Multiplex PCR, real-time PCR, and LAMP and analyze the advances and drawbacks of each method to provide a comprehensive view of pasteurellosis diagnosis.