Optimization of culture conditions and study on naphthalene and dibenzofuran degradation abilities of Paenibacillus naphthalenovorans 4B1
Vol. 133 No. 1A (2024), Original Article
Vol. 133 No. 1A (2024)

Optimization of culture conditions and study on naphthalene and dibenzofuran degradation abilities of Paenibacillus naphthalenovorans 4B1

Original Article
https://doi.org/10.26459/hueunijns.v133i1A.7391
Published 2024-03-29
Le Thi Ha Thanh*
University of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam
Nguyen Thi Thanh Thuy
Nguyen Binh Khiem High School, Chu Se, Gia Lai, Vietnam
Le Thi Van Anh
University of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam
Nguyen Thi Kieu Giang
University of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam
Hoang Duong Thu Huong
University of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam
PDF (Vietnamese)

Keywords

naphthalene
dibenzofuran
degradation
Paenibacillus naphthalene
dibenzofuran
phân hủy
Paenibacillus

How to Cite

1.
Hà Thanh LT, Thuỷ NTT, Anh LTV, Giang NTK, Hương HDT. Optimization of culture conditions and study on naphthalene and dibenzofuran degradation abilities of Paenibacillus naphthalenovorans 4B1. hueuni-jns [Internet]. 2024Mar.29 [cited 2024Nov.29];133(1A):31-42. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7391
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Abstract

The pollution of polycyclic aromatic hydrocarbon compounds caused by herbicide residues contaminated with dioxin from the war and other wastes from industrial activities has left severe consequences to human health and the environment. Bioremediation using microorganisms capable of decomposing pollutant compounds exhibits more advantages than physicochemical treatment methods as it is highly efficient, economically feasible, sustainable, and eco-friendly. In this study, Paenibacillus naphthalenovorans strain 4B1, isolated from dioxin-contaminated soil in Vietnam, was optimized the culture conditions and evaluated its abilities to degrade dibenzofuran and naphthalene. The results revealed that strain 4B1 showed the best growth on mineral salts medium supplemented with dibenzofuran 1250 mg/L or naphthalene 750 mg/L, pH 7.0, incubated at 45°C and 180 rpm with a cell density of 9,42 × 107 và 5,6 × 107 CFU/mL, respectively. Gas chromatography analysis of the substrate concentrations indicated that strain 4B1 could degrade 79.76% of 1250 mg/L dibenzofuran and 83.03% of 750 mg/L naphthalene after 72 hours of incubation at optimal conditions. These results make Paenibacillus naphthalenovorans 4B1 a potential candidate for application in bioremediation of environments contaminated with polycyclic aromatic hydrocarbon compounds.

https://doi.org/10.26459/hueunijns.v133i1A.7391
PDF (Vietnamese)

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