Isolation and quantification of major saponin ginsenoside Re from hairy root cultures of Panax vietnamensis Ha et Grushv by HPLC

Abstract

From the 80% ethanol extract of the hairy root cultures of Panax vietnamensis, a main dammarane-type saponin was isolated by using the chromatographic method. The isolate was identified as ginsenoside Re (1) via an extensive analysis by using NMR and MS, as well as comparison with published data. A quantitative procedure using HPLC with a UV detector has been developed to determine the ginsenoside Re content from the hairy roots of cultured P. vietnamensis, and the procedure was also validated according to the ICH guidelines in terms of accuracy, repeatability, linearity, and selectivity. The determination was carried out by using a C18 Shimadzu Shim-pack GIST (4.6 × 250 mm; 5 µm) column as a stationary phase with a gradient solvent system of acetonitrile-water as eluant. The flow rate was set at 1.0 mL/min and UV detection at 196 nm. This is the first report on the isolation and quantitative analysis of ginsenoside Re in the samples of P. vietnamensis hairy roots.