Effect of extraction temperature on content and in vitro antioxidant activity of polysaccharide from Gymnopetalum cochinchinense

Abstract

The effect of extraction temperature on the in vitro antioxidant activity of polysaccharides from Gymnopetalum cochinchinense was investigated. The results show that at an extraction temperature of 80 °C, the antioxidant activity of the polysaccharides was the highest. The total antioxidant capacity of the PS-W-T80 sample was 0.2726 ± 0.0003 mg GA/g or 0.1776 ± 0.0001 mg AS/g. Furthermore, its IC₅₀ values in the DPPH and ABTS radical scavenging models were 0.83 and 3.30 mg/mL, respectively. Fourier transform infrared spectroscopy analysis indicates that the polysaccharides in the PS-W-T80 sample exhibit characteristic absorption peaks commonly found in polysaccharide structures. The average molecular weight of the extracted polysaccharide is approximately 5.56 × 10⁴ Da. These results show that the polysaccharide in PS-W-T80 from G. cochinchinense is a potential antioxidant agent.