Simultaneous detection of paracetamol, chlorpheniramine maleate, cinnarizine, loratadine, and cetirizine hydrochloride in preparations derived from medicinal herbs by using high-performance liquid chromatography

Abstract

This study was conducted to develop a method for simultaneous quantification of paracetamol (PAR), chlorpheniramine maleate (CPM), cinnarizine (CET), loratadine (LOR), and cetirizine hydrochloride (CIN) in preparations derived from medicinal herbs by using high-performance liquid chromatography (HPLC) with a PDA detector. The analytical method was performed under the following chromatographic conditions: InertSustainTM C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: a mixture of ACN (A) and 5 mM NaH₂PO₄ (pH = 2.3) (B) according to a gradient program; flow rate: 1.0 mL/min; sample injection volume: 20 μL; column temperature: 40 °C; detection wavelength: 245 nm for Paracetamol, 225 nm for chlorpheniramine maleate, 230 nm for cetirizine hydrochloride, 254 nm for loratadine, and 253 nm for cinarizin. The analytical method was validated according to ICH and AOAC guidelines. The detection limits of PAR, CPM, CET, LOR, and CIN were 2.00, 2.00, 1.75, 1.00, and 0.50 µg/mL, respectively. The developed procedure was applied to analyze 32 samples of traditional medicinal products collected from the local markets.