Isolation and characterisation of phosphate-solubilising bacteria from serpentine soils in Yen Bai Province
Vol. 134 No. 1S-3 (2025), Special Issue: CBA 2025
Vol. 134 No. 1S-3 (2025)

Isolation and characterisation of phosphate-solubilising bacteria from serpentine soils in Yen Bai Province

Special Issue: CBA 2025
https://doi.org/10.26459/hueunijns.v134i1S-3.7932
Published 2025-12-26
Mai Thi Ngoc Dinh
Faculty of Biotechnology, Chemistry and Environmental Engineering, Phenikaa University, Hanoi 12116, Vietnam
Dinh Quoc Nguyen
External Engagement Office, Phenikaa University, Hanoi 12116, Vietnam
Hai The Pham
VNU University of Science, 334 Nguyen Trai St., Thanh Xuan, Hanoi, Vietnam
Dinh Thi Phuong Tran
VNU University of Science, 334 Nguyen Trai St., Thanh Xuan, Hanoi, Vietnam
Thao Kim Nu Nguyen
VNU University of Science, 334 Nguyen Trai St., Thanh Xuan, Hanoi, Vietnam
Nguyen Hong Minh*
Bioresource Research Center, Phenikaa University, Hanoi 12116, Vietnam
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Keywords

Phosphate solubilizing bacteria
Serpentine soil
Burkholderia sp.
Biofertilizer

How to Cite

1.
Dinh MTN, Nguyen DQ, Pham HT, Tran DTP, Nguyen TKN, Nguyen HM. Isolation and characterisation of phosphate-solubilising bacteria from serpentine soils in Yen Bai Province. hueuni-jns [Internet]. 2025Dec.26 [cited 2025Dec.27];134(1S-3):61-7. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7932
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Abstract

Phosphate-solubilising bacteria (PSB) contribute to plant phosphorus nutrition by transforming insoluble phosphates into bioavailable forms. In our work, PSB were isolated from serpentine soils in Yen Bai Province, Vietnam—an environment typified by low nutrient availability and elevated metal content. Forty-two bacterial isolates with phosphate-solubilising traits were screened in the NBRIP medium. Among them, strain VW132 demonstrated the highest solubilisation capacity and was taxonomically identified as Burkholderia sp. via 16S rRNA gene analysis. Strain VW132 reached maximum phosphate solubilisation of 359 mg/L when cultivated under optimised conditions, i.e, ammonium sulfate and glucose as nitrogen and carbon sources, 1% NaCl, and pH 7. However, phosphate solubilisation markedly declined at NaCl concentrations exceeding 1%, suggesting reduced salt tolerance. The relationship between soluble phosphate concentration and biofilm development was also investigated. Results show enhanced biofilm formation with increasing levels of K2HPO4, indicating a phosphate-dependent biofilm response.

https://doi.org/10.26459/hueunijns.v134i1S-3.7932
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