Effect of Culture Conditions on Production Laccase from Trametes versicolor and Partial Purification Extracellular Enzyme
Vol. 134 No. 1S-2 (2025), Special Issue: CBA 2025
Vol. 134 No. 1S-2 (2025)

Effect of Culture Conditions on Production Laccase from Trametes versicolor and Partial Purification Extracellular Enzyme

Special Issue: CBA 2025
https://doi.org/10.26459/hueunijns.v134i1S-2.7966
Published 2025-12-17
Chi Cuong Doan
The University of Danang - University of Science and Education, Danang 550000, Viet Nam
Duc Thang Bui
The University of Danang - University of Science and Education, Danang 550000, Viet Nam
Minh Nhat Dang
The University of Danang - University of Science and Technology, Danang 550000, Viet Nam
Chau Tuan Vo
The University of Danang - University of Science and Education, Danang 550000, Viet Nam
Thi Diem Pham
The University of Danang - University of Science and Education, Danang 550000, Viet Nam
Nguyen Thi Bich Hang*
The University of Danang - University of Science and Education, Danang 550000, Viet Nam
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Keywords

Laccase
Trametes versicolor
purification
enzyme activity

How to Cite

1.
Doan CC, Bui DT, Dang MN, Vo CT, Pham TD, Nguyen TBH. Effect of Culture Conditions on Production Laccase from Trametes versicolor and Partial Purification Extracellular Enzyme. hueuni-jns [Internet]. 2025Dec.17 [cited 2025Dec.27];134(1S-2):71-80. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7966
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Abstract

Laccase is a multicopper oxidase enzyme which oxidizes a wide range of substrates, including phenols, ketones, phosphates, ascorbate, amines, and lignin. This study was conducted to evaluate the effects of medium composition, cultivation time, and culture method on laccase production by Trametes versicolor. The results showed that the optimal medium for laccase production consisted of potato extract, glucose, KH₂PO₄, MgSO₄, NH₄⁺, Cu²⁺, and ABTS. Under these conditions, laccase activity reached 2.858 U/mL after 8 days of cultivation. Additionally, the study investigated the effects of different precipitation agents on the efficiency of laccase purification from the culture broth. The ratios of enzyme solution to organic solvents (ethanol, acetone) varied from 1:1 to 1:5 (v/v), and the saturation level of ammonium sulfate ranged from 40% to 80%. The results indicated that ethanol was the most effective precipitating agent, with the highest laccase yield obtained at a ratio of enzyme to ethanol of 1:3 (v/v) and a treatment time of 45 minutes. The molecular weight of the purified laccase was approximately 100 kDa as determined by SDS-PAGE analysis. These findings highlight the potential of T. versicolor culture broth as a source for laccase enzyme production, supporting its application in industrial bioprocesses such as bioremediation, food processing, and the development of sustainable biotechnology solutions.

https://doi.org/10.26459/hueunijns.v134i1S-2.7966
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