Evaluation of genetic diversity of 12 gerbera varieties (Gerbera L.) based on morphology and RAPD marker
PDF

Keywords

Gerbera, genetic diversity, morphological characters, molecular marker, RAPD marker. Gerbera
genetic diversity
morphological characteristics
molecular marker
RAPD marker

How to Cite

1.
Nguyen TA, Tran TTD, Quan NG, Le VT, Trinh NA. Evaluation of genetic diversity of 12 gerbera varieties (Gerbera L.) based on morphology and RAPD marker. hueuni-jns [Internet]. 2025Dec.17 [cited 2025Dec.27];134(1S-2):151-64. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7969

Abstract

This study focuses on evaluating the genetic diversity of 12 Gerbera varieties from farms in Da Lat city (Lam Dong province) based on 9 morphological characteristics and 10 RAPD primers. A higher different level between varieties was observed by using RAPD markers compared to morphological markers. A total of 110/135 polymorphic bands were generated, which occupied 12.2%. The average of PIC and Rp value are 0.48 and 11.39, respectively. The genetic similarity coefficient based on morphological analysis and RAPD data was 0.98-0.99 and 0.46-0.82, respectively, which showed that RAPD primers are useful for detecting genetic polymorphism. The results from morphological data showed that XB, HX and DX2 varieties are suitable for the cut flower market; meanwhile, DD1 and DD2 varieties are characterized by large flower size, DD2 and DX2 have long and wide petals. The RAPD analysis results indicated that OPE02, OPE08 and OPE13 generated a high polymorphic rate (100%), while OPG16 primer showed the lowest polymorphism percentage (52.9%). This study demonstrates that the combination of phenotypic and genotypic methods provides valuable insights into the understanding of genetic diversity among species.

https://doi.org/10.26459/hueunijns.v134i1S-2.7969
PDF

References

  1. Hansen HV. A taxonomic revision of the genus Perdicium (Compositae -Mutisieae). Nordic Journal of Botany. 1985;5(6):543-6.
  2. Gantait S, Mandal N, Bhattacharyya S, Das PK. Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell, Tissue and Organ Culture (PCTOC). 2011;106(3):485-93.
  3. de Pinho Benemann D, Arge LWP, Barros WS, Bianchi VJ, Segeren MI, Peters JA. Genetic divergence among Gerbera spp. genotypes based on morphological traits. Journal of Agricultural Science. 2013;5(5):35-45.
  4. Kurnia Y, Rika M, Ridho K, editors. Genetic and Phenotypic Variabilities of Gerbera (Gerbera jamesonii H. Bolus) Generated from Hybridization. Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020); 2021. Dordrecht: Atlantis Press.
  5. Filliettaz A. Melhoramento Genético de Plantas Ornamentais. Biologico, São Paulo. 2007;69(2):95.
  6. Mohammadi SA, Prasanna BM. Analysis of genetic diversity in crop plants—salient statistical tools and considerations. Crop Science. 2003;43(4):1235-48.
  7. Borém A, Miranda GV, Fritsche-neto R. Melhoramento de plantas. São Paulo: Oficina de Textos; 2021.
  8. Kumar R. Studies on genetic variability in gerbera (Gerbera jamesonii Bolus ex. Hooker F.). Journal of Horticultural Sciences. 2013;8(1):111-3.
  9. Hayden MJ, Tabone TL, Nguyen TM, Coventry S, Keiper FJ, Fox RL, et al. An informative set of SNP markers for molecular characterisation of Australian barley germplasm. Crop & Pasture Science. 2009;61(1):70-83.
  10. Mongkolporn O, Dokmaihom Y, Kanchana-Udomkan C, Pakdeevaraporn P. Genetic purity test of F1 hybrid Capsicum using molecular analysis. The Journal of Horticultural Science and Biotechnology. 2004;79(3):449-51.
  11. Niklas-Nowak A, Olszewska D. Molekularna identyfikacja ustalonych i mieszańcowych form Capsicum annuum L. z wykorzystaniem markerów RAPD. Zeszyty Problemowe Postępów Nauk Rolniczych. 2018:592.
  12. Arafa RA, Prohens J, Solberg S, Plazas M, Rakh M. Breeding and Genome Mapping for Resistance to Biotic Stress in Eggplant. In: Kole C, editor. Genomic Designing for Biotic Stress Resistant Vegetable Crops. Cham: Springer International Publishing; 2022. p. 147-87.
  13. Rasphone S, Ho THN, Dang TL, Nguyen BLQ, Truong HTH. Genetic diversity analysis of black pepper (Piper spp.) with RAPD markers. Hue University Journal of Science: Natural Science. 2022;131(1D):49-59.
  14. Slameto S. Genetic diversity and molecular analysis using RAPD markers of banana cultivars in the five regions of East Java, Indonesia. Biodiversitas Journal of Biological Diversity. 2023;24(9):5035-5043.
  15. Ince AG, Karaca M, Onus AN. Polymorphic Microsatellite Markers Transferable Across Capsicum Species. Plant Molecular Biology Reporter. 2010;28(2):285-91.
  16. Chung YM, Kim HA, Kim KY, Park SW, Yi YB, Lee JH, et al. Morphologicol Characteristics and Genetic Variation of Gerbera (Gerbera hybrida Hort). Journal of Plant Biotechnology. 2001;3(3):145-149.
  17. Mata TLD, Segeren MI, Fonseca AS, Colombo CA. Genetic divergence among gerbera accessions evaluated by RAPD. Scientia Horticulturae. 2009;121(1):92-6.
  18. Saidi A, Hajkazemian M, Emami SN. Evaluation of genetic diversity in gerbera genotypes revealed using SCoT and CDDP markers. Polish Journal of Natural Sciences. 2020;35(1):21-34.
  19. Maitra S, Shankar T, Sairam M, Pine S. Evaluation of gerbera (Gerbera jamesonii L.) cultivars for growth, yield and flower quality under protected cultivation. Indian Journal of Natural Sciences. 2020;10(60):20271-20276.
  20. Chu H, Jeong JC, Kim W-J, Chung DM, Jeon HK, Ahn YO, et al. Expression of the sweetpotato R2R3‐type IbMYB1a gene induces anthocyanin accumulation in Arabidopsis. Physiologia plantarum. 2013;148(2):189-99.
  21. Rohlf FJ. Statistical power comparisons among alternative morphometric methods. American Journal of Physical Anthropology. 2000;111(4):463-78.
  22. Loridon K, McPhee K, Morin J, Dubreuil P, Pilet-Nayel ML, Aubert G, et al. Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theoretical and Applied Genetics, 2005, 111: 1022-1031.
  23. Prevost A, Wilkinson MJ. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics. 2005;111(6):1022-31.
  24. Prajapati P, Singh A, Patel NL, Singh D, Srivastav V. Evaluation of genetic diversity in different genotypes of Gerbera jamesonii Bolus using random amplified polymorphic DNA (RAPD) markers. African Journal of Biotechnology. 2015;13(10).
  25. Hong Y, Bai XX, Sun W, Jia FW, Dai SL. The Numerical Classification of Chrysanthemum Flower Color Phenotype. Acta Horticulturae Sinica. 2012;39(7):1330-1340.
  26. Grotewold E. The genetics and biochemistry of floral pigments. Annual Review of Plant Biology. 2006;57(Volume 57, 2006):761-80.
  27. Zhou Y, Yin M, Abbas F, Sun Y, Gao T, Yan F, et al. Classification and association analysis of Gerbera (Gerbera hybrida) flower color traits. Frontiers in Plant Science. 2022;Volume 12 – 2021.
  28. Chen HX, Liu MY, Lu CP. Effect of pigment distribution on the color expression of Gerbera hybrida petal. 2010;36:165–168.
  29. Sudré CP, Cruz CD, Rosdrigues R, Riva EM, do Amaral JAT, da Silva DJH, et al. Multicategoric variables for determining the genetic divergence among sweet and chilli peppers. Horticultura Brasileira. 2006;24:88-93.
  30. Bertini CHCM, Pinhero EA, Nobrega GN, Duarte JML. Agronomic performance and genetic divergence of coriander genotypes. Revista Ciência Agronômica. 2010;41.3:409.
  31. Amorim E, Ramos NP, Ungaro MRG, Kiih TAM. Genetic divergence in sunflower genotypes. Ciência e Agrotecnologia. 2007;31:1637-1644.
  32. Roldán-Ruiz I, Dendauw J, Van Bockstaele E, Depicker A, De Loose M. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding. 2000;6(2):125-34.
  33. Rezende RKS, Paiva LV, Chalfun junior A, Torga PP, Masetto TE. Genetic divergence among cultivars of gerbera using RAPD markers. Ciencia Rural. 2009;39:2435-2440.
  34. Gong L, Deng Z. Selection and application of SSR markers for variety discrimination, genetic similarity and relation analysis in gerbera (Gerbera hybrida). Scientia Horticulturae. 2012;138:120-7.
  35. D’Imperio M, Viscosi V, Scarano M-T, D’Andrea M, Zullo BA, Pilla F. Integration between molecular and morphological markers for the exploitation of olive germoplasm (Olea europaea). Scientia Horticulturae. 2011;130(1):229-40.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2025 Array