Abstract
Arthrospira platensis, a filamentous cyanobacterium rich in nutrients, holds great potential for applications in aquaculture, biotechnology, and functional food products. This study investigates the impact of three organic carbon sources—glucose, sodium acetate, and glycerol—on the growth and biochemical profile of A. platensis under mixotrophic conditions. Glucose (1 g/L) and sodium acetate (0.5 g/L) significantly promoted growth rates (0.38 ± 0.04 and 0.37 ± 0.02 day⁻¹, respectively) and biomass production. Conversely, glycerol inhibited growth across all concentrations. While all carbon sources enhanced lipid accumulation—most notably at 2 g/L sodium acetate (6.78 ± 1.16 mg/mL/day)—they simultaneously reduced protein content. These results support the strategic use of carbon sources to optimize A. platensis cultivation for high-value biomass production in aquaculture and bioindustry applications.
References
- Cheirsilp B, Torpee S. Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed-batch cultivation. Bioresour Technol. 2012;110:510-516.
- Ciferri O. Spirulina, the edible microorganism. Microbiol Rev. 1983;47(4):551-578.
- Chojnacka K, Noworyta A. Evaluation of Spirulina sp. growth in photoautotrophic, heterotrophic and mixotrophic cultures. Enzyme Microb Technol. 2004;34(5):461-465.
- Chen CY, Yeh KL, Aisyah R, Lee DJ, Chang JS. Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review. Bioresour Technol. 2011;102(1):71-81.
- González-Fernández C, Ballesteros M. Linking microalgae and cyanobacteria culture conditions and key-enzymes for carbohydrate accumulation. Biotechnol Adv. 2012;30(6):1655-1661.
- Jaki B, Orjala J, Sticher O. A novel extracellular diterpenoid with antibacterial activity from the cyanobacterium Nostoc commune. J Nat Prod. 1999;62(3):502-503.
- Jensen EL, Yangüez K, Carrière F, Gontero B. Storage compound accumulation in diatoms as response to elevated CO₂ concentration. Biology. 2019;9(1):5.
- Li Y, Horsman M, Wang B, Wu N, Lan CQ. Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol. 2008;81(4):629-636.
- Markou G, Nerantzis E. Microalgae for high-value compounds and biofuels production: a review with focus on cultivation under stress conditions. Biotechnol Adv. 2013;31:1532-1542.
- Mishra SK, Suh WI, Farooq W, Moon M, Shrivastav A, Park MS, et al. Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method. Bioresour Technol. 2014;155:330-333.
- Markou G, Kougia E, Kefalogianni I, Tsagou V, Arapoglou D, Chatzipavlidis I. Effect of glycerol concentration and light intensity on growth and biochemical composition of Arthrospira (Spirulina) platensis: a study in semi-continuous mode with non-aseptic conditions. Appl Sci. 2019;9(21):4703.
- Marquez FJ, Nishio N, Nagai S, Sasaki K. Enhancement of biomass and pigment production during growth of Spirulina platensis in mixotrophic culture. J Chem Technol Biotechnol. 1995;62:159-164.
- Matsudo MC, Moraes FA, Bezerra RP, Arashiro RE, Sato S, Carvalho JCM. Use of acetate in fed-batch mixotrophic cultivation of Arthrospira platensis. Ann Microbiol. 2015;65(3):1721-1728.
- Patterson GML, Larsen LK, Moore RE. Bioactive natural products from blue-green algae. J Appl Phycol. 1994;6(2):151-157.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Copyright (c) 2025 Array