Selection of Chlorella sp. from shrimp pond wastewater and determination of the optimal inoculum density for biomass production
Vol. 135 No. 1S-1 (2026), Special Issue: IFB 2025
Vol. 135 No. 1S-1 (2026)

Selection of Chlorella sp. from shrimp pond wastewater and determination of the optimal inoculum density for biomass production

Special Issue: IFB 2025
https://doi.org/10.26459/hueunijns.v135i1S-1.8072
Published 2026-06-10
Ngoc Thanh Tam Huynh
Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
Truong Lam Pham
Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
Thi Pha Nguyen
Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
Tran Huu Hau*
Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
PDF (Vietnamese)

Keywords

biomass cultivation
Chlorella
microalgae
propagation Chlorella
nuôi sinh khối
nhân giống
vi tảo

How to Cite

1.
Huỳnh NTT, Phạm TL, Nguyễn TP, Tran HH. Selection of Chlorella sp. from shrimp pond wastewater and determination of the optimal inoculum density for biomass production. hueuni-jns [Internet]. 2026Jun.10 [cited 2026Jun.12];135(1S-1):45-56. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/8072
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Abstract

Microalgae is a rich source of protein, lipids, minerals, and vitamins. They could be employed as an extra food source for animals. However, there are still a lot of problems with selecting and culturing strains that have a lot of biomass potential. The study selected the TV1 strain from shrimp pond effluent because it grew the fastest among the seven microalgae strains isolated on Conway medium. Morphologically, the cells are spherical, with a diameter of 3 to 5 µm. The 18S rRNA gene sequence analysis and its comparison with the NCBI database classified the TV1 strain as belonging to the Chlorophyceae class and the Chlorella genus. Chlorella sp. TV1 is cultured in three stages. The primary culture propagation was carried out at a cell density of 0.313x106 cells/mL. The second and third subculture stages were carried out over 9 days, with initial cell densities of 0.634×106 and 0.452×106 cells/mL, respectively. The study selected the Chlorella sp. strain TV1 from shrimp pond wastewater, and finding a beneficial way to cultivate it will help produce a large amount of microalgae biomass. Further research is needed to improve animal husbandry environments and determine the effectiveness of wastewater treatment, with the goal of using it in circular agriculture.

https://doi.org/10.26459/hueunijns.v135i1S-1.8072
PDF (Vietnamese)

References

  1. Borowitzka MA. The ‘stress’ concept in microalgal biology-homeostasis, acclimation and adaptation. J Appl Phycol. 2018;30(5):2815-2825.
  2. Tran CB. Technical efficiency of Chlorella sp. algae biomass culture using wastewater from Pangasianodon hypophthalmus ponds. CTU J Sci. 2013;28:157-162.
  3. Tran CB, Le TQE, Pham HN, Nguyen XL, Nguyen MC. Usage of wastewater from Pangasianodon hypophthalmus ponds to culture Chlorella sp. CTU J Sci. 2015;39:90-96.
  4. Nguyen TKH, Dang TT, Nguyen CT, Nguyen TPO. Study on potential for nitrate and phosphate absorbance of microlagae isolated from domestic wastewater. CTU J Sci. 2021;57(4):73-81.
  5. Wang Y, Zhang Y, Wang J. Effect of pH on growth and lipid content of Chlorella vulgaris cultured in biogas slurry. Chin J Biotechnol. 2010;26(8):1074-1079.
  6. Vo TKT, Nguyen DT, Vo TLA, Pham HH. Application of Chlorella sp. and Daphnia sp. for treating organic waste derived from swine wastewater after UASB system usage. Acad J Biol. 2012;34(3se):145-153.
  7. Nguyen TTX, Dang KH, Vo TT. Possibility of anaerobic digester effluent treatment through microalgae Chlorella vulgaris cultivation in orientation of biofuel production. Univ Danang J Sci Technol. 2014;3(76):83-86.
  8. Becker EW. Micro-algae as a source of protein. Biotechnol Adv. 2007;25(2):207-210.
  9. Gouveia L, Oliveira AC. Microalgae as a raw material for biofuels production. J Ind Microbiol Biotechnol. 2009;36(2):269-274.
  10. Sayeda MA, Ali GH, El-Baz FK. Potential production of omega fatty acids from microalgae. Int J Pharm Sci Rev Res. 2015;34(2):210-215.
  11. Abdel-Moatamed BR, El-Fakhrany AEM, Elneairy NA, Shaban MM, Roby MH. The impact of Chlorella vulgaris fortification on the nutritional composition and quality characteristics of beef burgers. Foods. 2024;13(12):1945.
  12. Georgiou D, Charisis A, Exarhopoulos S, Papapanagiotou G, Samara C, Chatzidoukas C, Kalogianni EP. Comparative evaluation of Chlorella vulgaris and Chlorella sorokiniana for food-related applications. Waste Biomass Valor. 2025;1-15.
  13. Nguyen TTT, Mac NB, Tran NN. Influence of nutrient media and salinity on growth of microalgae Nannochloropsis oculata. Hue Univ J Sci Agric Rural Dev. 2021;130(3A):13-23.
  14. Richmond A, Hu Q. Handbook of microalgal culture: Applied phycology and biotechnology. 2nd ed. Chichester (UK): Wiley-Blackwell; 2013.
  15. Santhanam P, Kumar SD, Ananth S, Jeyanthi S, Sasirekha R, Premkumar C. Effect of culture conditions on growth and lipid content of marine microalga Nannochloropsis sp. strain (PSDK11). Indian J Geo-Mar Sci. 2017;46(11):2332-2338.
  16. Andersen RA, Brett RW, Potter D, Sexton JP. Phylogeny of the Eustigmatophyceae based upon 18s rDNA, with emphasis on Nannochloropsis. Protist. 1998;149(1):61-74.
  17. Baroni ÉG, Yap KY, Webley PA, Scales PJ, Martin GJO. The effect of nitrogen depletion on the cell size, shape, density and gravitational settling of Nannochloropsis salina, Chlorella sp. (marine) and Haematococcus pluvialis. Algal Res. 2019;39:101454.
  18. Singh V, Laura JS. Spectral monitoring and quantification of Chlorella sorokiniana for biomass production. Ann Biol. 2020;36(1):43-47
  19. Tasnim N, Karmakar D, Hasan R, Islam R, Hossain S, Shaikh AA, Karim R. Effect of light intensity and pH on cell density assessed by spectrophotometry for the unicellular algae Chlorella vulgaris. Am J Plant Sci. 2023;14(4):472-481.
  20. Do TBXL, Nguyen TK, Vu XT, Dao BN, Nguyen HH. A simple, efficient and universal method for the extraction of genomic DNA from bacteria, yeasts, molds and microalgae suitable for PCR-based applications. Vietnam J Sci Technol Eng. 2017;59(4):66-74.
  21. Tran TXM, Nguyen VB, Nguyen TP. Nguyen TL. Isolation and identification of thraustochytrid heterotrophic microalga for production of carotenoids. CTU J Sci. 2015;37:57-64.
  22. Tran SN, Huynh TNH, Pham TTN. Development of Chlorella sp. in heterotrophic cultivation. CTU J Sci. 2017;50:127-132.
  23. Zhang Q, Zhan J, Hong Y. The effects of temperature on the growth, lipid accumulation and nutrient removal characteristics of Chlorella sp. HQ. Desalin Water Treat. 2016;57(22):104030-10408.
  24. Wang CA, Onyeaka H, Miri T, Soltani F. Chlorella vulgaris as a food substitute: Applications and benefits in the food industry. J Food Sci. 2024;89(12):8231-8247.
  25. De Schryver P, Vadstein O. Ecological theory as a foundation to control pathogenic invasion in aquaculture. ISME J. 2014;8(11):2360-2368.
  26. Tsai MT. Study on the lipid production from microalgal cultures and producing biodiesel from the microalgal lipid [Master’s thesis]. Hsinchu (Taiwan): National Chiao Tung University; 2009.
  27. Ghoul M, Mitri S. The ecology and evolution of microbial competition. Trends Microbiol. 2016;24(10):833-845.
  28. Valiei A, Dickson AM, Aminian-Dehkordi J, Mofrad MRK. Bacterial community dynamics as a result of growth-yield trade-off and multispecies metabolic interactions toward understanding the gut biofilm niche. BMC Microbiol. 2024;24:441.
  29. Zhang L, Lu HF, Zhang YH, Ma SS, Li BM, Liu ZD. Effects of strain, nutrients concentration and inoculum size on microalgae culture for bioenergy from post hydrothermal liquefaction wastewater. Int J Agric Biol Eng. 2017;10(2):194-204.
  30. Bohutskyi P, Kligerman DC, Byers N, Nasr LK, Cua C, Chow S. Effects of inoculum size, light intensity, and dose of anaerobic digestion centrate on growth and productivity of Chlorella and Scenedesmus microalgae and their poly-culture in primary and secondary wastewater. Algal Res. 2016;19:278-290.
  31. Abdul-Sani ER, Chin GJWL, Yong WTL, Misson M. Optimization of inoculum cell concentration for enhanced lipid production in laboratory-scale cultivation of the marine microalga Chlorella sp. for biofuel applications. Front Energy Res. 2024;12:1490421.
  32. Cutler TD, Zimmerman JJ. Ultraviolet irradiation and the mechanisms underlying its inactivation of infectious agents. Anim Health Res Rev. 2011;12(1):15-23.
  33. Górny RL, Gołofit-Szymczak M, Pawlak A, Ławniczek-Wałczyk A, Cyprowski M, Stobnicka A, Płocińska M. Effectiveness of UV-C radiation in inactivation of microorganisms on materials with different surface structures. Ann Agric Environ Med. 2024;31(2):287-293.
  34. Deruyck B, Nguyen TNK, Decaestecker E, Muylaert K. Modeling the impact of rotifer contamination on microalgal production in open pond, photobioreactor and thin layer cultivation systems. Algal Res. 2019;38:101398.
  35. Di Caprio F. Methods to quantify biological contaminants in microalgae cultures. Algal Res. 2020;49:101943.
  36. Laezza C, Salbitani G, Carfagna S. Fungal contamination in microalgal cultivation: Biological and biotechnological aspects of fungi-microalgae interaction. J Fungi. 2022;8(10):1099.
  37. Assuncao FF de O, Nascimento É, Chaves L, da Silva AMH, Martinez R, Guirro RR de J. Inhibition of bacterial growth through LED (light-emitting diode) 465 and 630 nm: In vitro. Lasers Med Sci. 2022;37(5):2439-2447.
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