Development of a diagnostic SCAR marker for Vibrio vulnificus causing ulcerative disease in marine fishes in Thua Thien Hue
Vol. 129 No. 1C (2020), Original Article
Vol. 129 No. 1C (2020)

Development of a diagnostic SCAR marker for Vibrio vulnificus causing ulcerative disease in marine fishes in Thua Thien Hue

Original Article
https://doi.org/10.26459/hueuni-jns.v129i1C.5780
Published 2020-06-30
Hoang Tan Quang
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Pham Thi Diem Thi
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Tran Thuy Lan
Institute of Biotechnology, Hue University, Road No. 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Pham Thi Hai Yen
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Tran Quang Khanh Van
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
Nguyen Duy Quynh Tram
University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, Vietnam
PDF (Vietnamese)

How to Cite

1.
Quảng HT, Thi PTD, Lan TT, Yến PTH, Vân TQK, Trâm NDQ. Development of a diagnostic SCAR marker for Vibrio vulnificus causing ulcerative disease in marine fishes in Thua Thien Hue. hueuni-jns [Internet]. 2020Jun.30 [cited 2024Nov.24];129(1C):99-108. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5780
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Abstract

Vibrio vulnificus is widespread on cultured fish with the ulcerative disease in the Thua Thien Hue province, Vietnam, and it is a potential human pathogen that can cause primary septicemia, wound infection, and gastroenteritis in susceptible individuals. Thus, this study is conducted to develop a molecular marker generated from random amplified polymorphic DNA (RAPD) for the rapid detection of V. vulnificus. Twenty random primers were tested by using the RAPD method to detect DNA polymorphisms among Vibrio species. The random primer OPA-09 generates a 1356-bp DNA fragment specific for V. vulnificus. This sequence was used to design a specific primer, and it was transferred to a sequence-characterized amplified region (SCAR) marker with a length of 938 bp (A9-938). The specific primers Vvul-1F/Vvul-938R amplify a unique DNA fragment in all isolates of V. vulnificus but not in the other tested Vibrio species. This marker is highly specific and can be used to identify the presence of V. vulnificus in seafood.

https://doi.org/10.26459/hueuni-jns.v129i1C.5780
PDF (Vietnamese)

References

  1. An NTT, Hải TN, Dung TT. Phân lập vi khuẩn Vibrio trên cá bớp (Rachycentron canadum) bị lở loét. Báo cáo tại: Hội nghị khoa học trẻ thủy sản toàn quốc lần thứ IV; 2013; Hồ Chí Minh.
  2. Li G, Zhao D, Huang L, Sun J, Gao D, Wang H, et al. Identification and phylogenetic analysis of Vibrio vulnificus isolated from diseased Trachinotus ovatus in cage mariculture. Aquaculture. 2006;261(1):17-25.
  3. Heng SP, Letchumanan V, Deng CY, Ab Mutalib NS, Khan TM, Chuah LH, et al. Vibrio vulnificus: An environmental and clinical burden. Frontiers in Microbiology. 2017;8:997-.
  4. Strom MS, Paranjpye RN. Epidemiology and pathogenesis of Vibrio vulnificus. Microbes and Infection. 2000;2(2):177-88.
  5. Ramazanzadeh R, Rouhi S, Shakib P, Shahbazi B, Bidarpour F, Karimi M. Molecular characterization of Vibrio cholerae Isolated from clinical samples in Kurdistan province, Iran. Jundishapur J Microbiol. 2015;8(5):e18119-e.
  6. Khanh NV, Linh NQ, Lan TT, Nhân LTT, Vân TQK, Cơ NTK, et al. Phân lập và sàng lọc các chủng Vibrio parahaemolyticus gây bệnh hoại tử gan tụy cấp tính trên tôm thẻ chân trắng nuôi tại Phong Điền, Thừa Thiên Huế bằng chỉ thị phân tử 16S rRNA. Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên. 2019;128(1E):47-58.
  7. Sairkar PK, Sharma A, Shukla NP. SCAR marker for identification and discrimination of Commiphora wightii and C. myrrha. Mol Biol Int. 2016;2016:1-10.
  8. Rossi CC, Pereira MF, Langford PR, Bazzolli DMS. A BOX-SCAR fragment for the identification of Actinobacillus pleuropneumoniae. FEMS Microbiology Letters. 2014;352(1):32-7.
  9. Kałużna M, Albuquerque P, Tavares F, Sobiczewski P, Puławska J. Development of SCAR markers for rapid and specific detection of Pseudomonas syringae pv. morsprunorum races 1 and 2, using conventional and real-time PCR. Appl Microbiol Biotechnol. 2016;100(8):3693-711.
  10. Yang Y, Hu J, Chen F, Ding D, Zhou C. Development of a SCAR marker-based diagnostic method for the detection of the Citrus target spot pathogen Pseudofabraea citricarpa. BioMed Research International. 2018;2018:1-5.
  11. Aggarwal R, Gupta S, Banerjee S, Singh V. Development of a SCAR marker for detection of Bipolaris sorokiniana causing spot blotch of wheat. Canadian journal of microbiology. 2011;57:934-42.
  12. Quang HT, Lan TT, Hai TTH, Yen PTH, Van TQK, Tung HT, et al. Genetic diversity and toxic genes analysis of Vibrio spp. isolated from white leg shrimp and marine fishes cultured in Tam Giang lagoon in Thua Thien Hue province, Vietnam. Indian Journal of Science & Technology. 2020;13(13):1412-22.
  13. Quảng HT, Lan TT, Thi PTD, Nhân LTT, Ngọc LMT, Trâm NDQ, et al. Xác định sự hiện diện của các gen độc tố ở các chủng Vibrio gây bệnh hoại tử gan tụy cấp tính trên tôm tại Thừa Thiên Huế. Tạp chí Khoa học, Đại học Huế: Khoa học Tự nhiên. 2020;129(1A):115-123.
  14. Anupama KP, Chakraborty A, Karunasagar I, Maiti B. Loop-mediated isothermal amplification assay as a point-of-care diagnostic tool for Vibrio parahaemolyticus: recent developments and improvements. Expert Review of Molecular Diagnostics. 2019;19(3):229-39.
  15. Kim HJ, Ryu JO, Lee SY, Kim ES, Kim HY. Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics. BMC Microbiology. 2015;15(1):239-50.
  16. Tsai YH, Chen PH, Yu PA, Chen CL, Kuo LT, Huang KC. A multiplex PCR assay for detection of Vibrio vulnificus, Aeromonas hydrophila, methicillin-resistant Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus agalactiae from the isolates of patients with necrotizing fasciitis. International Journal of Infectious Diseases. 2019;81:73-80.
  17. Alramahy SK. Molecular diagnostics for Vibrio cholera based on recA gene isolated from human in Diwaniyah city. Journal of Pharmaceutical Sciences and Research. 2018;10:1125-7.
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