Keywords
Bacillus tequilensis
bùn thải
tôm
protease sludge
shrimp
bùn thải
tôm
protease sludge
shrimp
How to Cite
1.
Nguyễn QL, Nguyễn Đức H, Trịnh TPT, Lê TKT, Lê CT. Characterization of extracellular protease produced by Bacillus tequilensis ON1. hueuni-jns [Internet]. 2022Mar.31 [cited 2024Nov.22];131(1A):109-17. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/6410
Abstract
A bacterial strain with casein hydrolytic activity was isolated from shrimp waste sludge from a culture pond at Phong Dien, Thua Thien Hue. Molecular identification using 16S rRNA nucleotide sequence shows a high similarity to Bacillus tequilensis on the NCBI database. Hence, the isolate was named B. tequilensis ON1. Extracellular protease activity value is 192,42 U·mL–1 after 24 h culture. Zymogram SDS-PAGE electrophoresis shows that the major extracellular protease has a molecular weight of 130 kDa. The enzyme exhibits maximal activity at 50 °C and pH 8. Protease activity increases in the presence of Ca2+ and Mg2+ ions, while Mn2+ inhibits protease activity. The protease activity was more active and stable in hydrophobic organic solvents.
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