Effects of storage duration and dilution ratio on the quality of Tre rooster sperm stored at 5 °C
Vol. 135 No. 1S-1 (2026), Special Issue: IFB 2025
Vol. 135 No. 1S-1 (2026)

Effects of storage duration and dilution ratio on the quality of Tre rooster sperm stored at 5 °C

Special Issue: IFB 2025
https://doi.org/10.26459/hueunijns.v135i1S-1.8058
Published 2026-06-10
Tran Thi Thanh Khuong*
Stem Cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
Lam Khanh Duy Nguyen
Stem Cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
Chi Cong Tran
Stem Cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
Huynh Chau Minh Nguyen
Stem Cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Cantho, Vietnam
Truong Thoai Kha Pham
GREENFEED Vietnam Joint Stock Company, Nhut Chanh, Ben Luc, Long An, Vietnam
PDF (Vietnamese)

Keywords

5°C
dilution ratio
storage duration
sperm
Tre chicken 5°C
bảo quản
gà Tre
thời gian
tinh trùng
tỷ lệ pha loãng

How to Cite

1.
Trần TTK, Nguyễn LKD, Trần CC, Nguyễn HCM, Phạm TTK. Effects of storage duration and dilution ratio on the quality of Tre rooster sperm stored at 5 °C. hueuni-jns [Internet]. 2026Jun.10 [cited 2026Jun.13];135(1S-1):113-21. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/8058
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Abstract

Semen storage for artificial insemination is an important technique for maintaining and rapidly increasing livestock populations. This study was conducted to determine the optimal dilution ratio and storage duration for Tre chicken (Gallus gallus domesticus) semen stored at 5 °C. Semen samples were collected, initially evaluated for quality, then diluted with SCE extender at four different ratios (1:4, 1:6, 1:8, and 1:10) and stored for 12 hours. Sperm quality was assessed at five time points (0, 3, 6, 9, and 12 hours) based on total motility, progressive motility, viability, membrane integrity, and acrosome integrity. The results showed that the 1:6 dilution ratio maintained sperm quality comparable to the 1:4 ratio (p > 0.05) and significantly higher than the 1:8 and 1:10 ratios (p < 0.05) for most evaluated parameters. After 9 hours of storage at the 1:6 dilution ratio, total motility, progressive motility, viability, membrane integrity, and acrosome integrity reached 56.92%, 38.74%, 69.38%, 59.97%, and 75.36%, respectively. These findings indicate that storing Tre chicken semen at 5 °C with a dilution ratio of 1:6 for up to 9 hours is an effective condition in this study, with practical significance for the conservation and breeding of indigenous chicken breeds in Vietnam.

https://doi.org/10.26459/hueunijns.v135i1S-1.8058
PDF (Vietnamese)

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