Selection and culture condition optimisation for amylase biosynthesis of marine fungal strains isolated from Nha Trang Bay and Van Phong Bay, Khanh Hoa province
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How to Cite

1.
Thuỷ PT, Sở Đinh T, Duy NV. Selection and culture condition optimisation for amylase biosynthesis of marine fungal strains isolated from Nha Trang Bay and Van Phong Bay, Khanh Hoa province. HueUni-JNS [Internet]. 2020Jun.30 [cited 2020Dec.5];129(1C):59-67. Available from: http://jos.hueuni.edu.vn/index.php/HUJOS-NS/article/view/5449

Abstract

Marine fungi play an important role in marine ecosystems and provide numerous valuable industrial products. This study screens for amylase-producing marine fungi strains and studies the appropriate culture conditions to obtain enzymes. From 160 isolated strains, all yeast strains do not exhibit amylase activity (0/112 strains), while the molds display amylase activity with a high rate at 70.8% (34/48 strains). Among these molds, the DM12M strain has the highest amylase activity (13.0 U/mL). Suitable culture conditions for amylase production of this strain are the modified Czapek–Dox medium supplemented with 1% soluble starch, 0.3% NaNO3, 0.5% NaCl, and 5 days incubation at pH 6.0 and 30 °C. Under these conditions, the amylase activity of the DM12M strain reaches 59.0 U/mL, approximately 4.5 times higher than that under pre-optimalization conditions. The DM12M strain has the ITS1-5.8S-ITS2 nucleotide sequence of 99.8–100% similarity to the strains of Aspergillus aculeatus; this reveals that it may belong to this species. The research provides scientific data on the factors affecting the ability of the marine fungi to produce extracellular amylase and opens up their potential for industrial application.

https://doi.org/10.26459/hueuni-jns.v129i1C.5449
PDF (Vietnamese)

References

  1. Vaidya S, Srivastava PK, Rathore P, Pragya R, Pandey AK. Amylases: a prospective enzyme in the field of biotechnology. The Journal of Applied Biosciences. 2015; 41: 1-18.
  2. Mohapatra B, Banerjee U, Bapuji M. Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp. Journal of Biotechnology. 1998;60:113–117.
  3. Li HF, Chi ZM, Wang XH, Duan XH, Ma LY, Gao LM. Purification and characterization of extracellular amylase from the marine yeast Aureobasidium pullulans N13d and its raw potato starch digestion. Enzyme and Microbial Technology. 2007;40:1006-1012.
  4. Sahoo K, Dhal N, Das R. Production of amylase enzyme from mangrove fungal isolates. African Journal of Biotechnology. 2014;13(46):4338-4346.
  5. Lanka S, Pydipally M, Latha JNL. Extraction and activity studies of industrially important enzymes from marine fusarium species isolated from Machilipatnam sea water, (a.p), India. European Journal of Pharmaceuticaland Medical Research. 2016;3(12): 254-258.
  6. Wang Y, Barth D, Tamminen A, Wiebe MG. Growth of marine fungi on polymeric substrates. BMC Biotechnology. 2016;16(3).
  7. Bình NTT. Nghiên cứu thu nhận enzym amylase của một số chủng nấm sợi phân lập từ rừng ngập mặn Cần Giờ [Luận văn]. Hồ Chí Minh: Trường Ðại học Sư phạm Thành phố Hồ Chí Minh; 2010.
  8. Lan PTN, Thành HN. Nghiên cứu nấm mốc có khả năng phân giải tinh bột phân lập từ ao nuôi tôm ở Đầm Sam – Chuồn, Thừa Thiên Huế Trường Đại học Khoa học, Đại học Huế. Tạp chí khoa học Đại học Huế. 2012;73(4):147-156.
  9. Bernfeld P. Amylases, α and β. Methods in Enzymology. 1955;1:149-58.
  10. Toju H, Tanabe AS, Yamamoto S, Sato H. High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples. PLoS ONE. 2012;7(7): e40863.
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 2013;30:2725-2729.
  12. Ominyi MC. Optimization of α-amylase and glucoamylase production from three fungal strains isolated from Abakaliki, Ebonyi State. European Journal of Experimental Biology. 2013;3(4):26-34.
  13. Mahapatra S, Banerjee D. Production and characterization of thermal acid amylase from A. aculeatus DBF9. Dynamic Biochemistry, Process Biotechnology and Molecular Biology. 2012;6(1): 109-112.
  14. Khokhar I, Mukhtar I,Mushtaq S. Comparative studies on the amylase and cellulase production of Aspergillus and Penicillium. Journal of Applied Sciences and Environmental Management. 2011;15(4):657-661.
  15. Poonsrisawat A, Paemanee A, Wanlapatit S, Piyachomkwan K, Eurwilaichitr L, Champreda V. Simultaneous saccharification and viscosity reduction of cassava pulp using a multi-component starch- and cell-wall degrading enzyme for bioethanol production. 3 Biotech. 2017;7(5):290.
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