CLONING AND EXPRESSION OF GENE ENCODING PROTEIN p65 FROM MYCOPLASMA HYOPNEUMONIAE IN E. COLI BL21(DE3)

Abstract

In this study, we successfullycloned and expressed the p65 gene encoding for p65 proteinof Mycoplasma hyopneumonia (M. hyopneumonia) isolated from pig lungs collected in Thua Thien Hue province, Vietnam. The p65 gene was amplified and cloned into pET200/D-TOPO vector and then transformed into the E. coli BL21 (DE3) strain. The results showed that the p65 gene segment was 936 bp, identical to the published p65 gene on GenBank (accession number: CP003131.1), encoding a polypeptide chain of 311 amino acid residues, identical to anamino acid sequence of a protein on GenBank (accession number: AAB67173.1). The denatured SDS-PAGE analysis showed a protein band of 37 kDa which corresponded to 6×His-p65 fusion protein.