In vitro propagation of Phalaenopsis sp. in Thua Thien Hue

Abstract

Flower stalk nodes of Phalaenopsis sp. were used as initial materials for in vitro propagation. The flower stalks sterilized with 0.3% HgCl₂ for 20 min exhibit the highest survival rate (66.67%). Flower stalk nodes were transferred to the MS medium, supplemented with BAP (0–5 mg·L^–1). The optimal medium for shoot regeneration is MS, supplemented with 4 mg·L^–1 BAP; all shoots are regenerated. The average shoot per explant is 4.22. The shoot multiplication results show that the MS supplemented with 2.5 mg·L^–1 BAP and 0.2 mg·L^–1 IBA is the best multiplication medium, providing 6.33 shoots per explant with an average shoot height of 2.75 cm. The healthy, 4–5 cm high shoots with 3–4 leaves were transferred to the MS medium, supplemented with 1.5 mg·L^–1 NAA and 0.5 g·L^–1 activated charcoal. All shoots formed roots, and the average number per shoot is 4.33. After rooting, the 5-cm high seedlings were grown on the sphagnum moss and sprayed with a 6 mg·L^–1 nanosilver solution every seven days. The best growth was observed after eight weeks.