Expression of nat05 encoding for a nattokinase in Bacillus subtilis BD170
Vol. 130 No. 1A (2021), Original Article
Vol. 130 No. 1A (2021)

Expression of nat05 encoding for a nattokinase in Bacillus subtilis BD170

Original Article
https://doi.org/10.26459/hueunijns.v130i1A.5785
Published 2021-03-10
Nguyen Thi Anh Thu
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Ho Thi Trang
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
PDF (Vietnamese)

Keywords

Bacillus subtilis
nattokinase
nat05
expression Bacillus subtilis
nattokinase
nat05
biểu hiện

How to Cite

1.
Thư NTA, Trang HT. Expression of nat05 encoding for a nattokinase in Bacillus subtilis BD170. hueuni-jns [Internet]. 2021Mar.10 [cited 2024Dec.22];130(1A):69-75. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5785
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Nattokinase, a serine protease found in the Japanese Natto product, can specifically dissolve fibrin fibers causing coagulation and is useful in endogenous thrombolysis. Currently, nattokinase is produced with the help of traditional fermentation and recombinant DNA technology approaches. In this study, we presents the optimization of time and the inducing substance (IPTG) concentration of gene nat05 encoding for a nattokinase in Bacillus subtilis BD170 with strong protease activity. After 10 hours’ cultivation, nat05 expresses 9.5 times higher than the control. At the IPTG concentration of 2 mM, nat05 exhibits the strongest activity, which is 10 times higher than the control.

https://doi.org/10.26459/hueunijns.v130i1A.5785
PDF (Vietnamese)

References

  1. Mỹ LTP. Nghiên cứu khả năng sinh tổng hợp Nattokinase của Bacillus subtilis phân lập từ thực phẩm chức năng natto [luận văn]. Huế: Đại học Khoa Học, Đại học Huế; 2015.
  2. Tuấn TQ, Ái LTT, Anh NQ, Trinh NT, Anh ĐTL, Hiệp ĐM, et al. Tạo dòng và biểu hiện vượt mức enzym phân hủy huyết khối nattokinase từ chủng Bacillus subtilis. Tạp chí Khoa học ĐHQGHN: Khoa học Tự nhiên và Công nghệ. 2014;30(6S).
  3. Chen PT, Chiang C-J, Chao Y-P. Strategy to approach stable production of recombinant nattokinase in Bacillus subtilis. Biotechnology Progress. 2007;23(4):808-13. DOI: https://doi.org/10.1021/bp070108j
  4. Liang X, Jia S, Sun Y, Chen M, Chen X, Zhong J, et al. Secretory Expression of Nattokinase from Bacillus subtilis YF38 in Escherichia coli. Molecular Biotechnology. 2007;37(3):187-94. DOI: https://doi.org/10.1007/s12033-007-0060-y
  5. Liang X, Zhang L, Zhong J, Huan L. Secretory expression of a heterologous nattokinase in Lactococcus lactis. Applied Microbiology and Biotechnology. 2007;75(1):95-101. DOI: https://doi.org/10.1007/s00253-006-0809-4
  6. Hương ĐT. Nghiên cứu điều kiện nuôi cấy và chiết enzyme protease từ vi khuẩn Bacillus subtilis natto [luận văn]. Hà Nội: Trường đại học Dược Hà Nội; 2013.
  7. Nguyen TT, Quyen TD, Le HT. Cloning and enhancing production of a detergent- and organic-solvent-resistant nattokinase from Bacillus subtilis VTCC-DVN-12-01 by using an eight-protease-gene-deficient Bacillus subtilis WB800. Microbial Cell Factories. 2013;12(1):79. DOI: https://doi.org/10.1186/1475-2859-12-79
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2021 Array