Polysaccharide extraction from Myxopyrum smilacifolium trunk and its antioxidant capacity
Vol. 132 No. 1D (2023), Original Article
Vol. 132 No. 1D (2023)

Polysaccharide extraction from Myxopyrum smilacifolium trunk and its antioxidant capacity

Original Article
https://doi.org/10.26459/hueunijns.v132i1D.7012
Published 2023-12-30
Le Trung Hieu*
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
Le Lam Son
letrunghieu.chem@gmail.com
Nguyen Dang Giang Chau
letrunghieu.chem@gmail.com
Nguyen Minh Nhung
Center for Measurement, Testing and Scientific information, Department of Science and Technology Thua Thien Hue province, Hue, Vietnam
Tran Thanh Minh
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
Ho Xuan Anh Vu
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
Le Thuy Trang
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
Nguyen Viet Thang
Faculty of Biology, Hue University of Sciences, Hue University, Hue, Vietnam
Nguyen Quang Man
University of Medicine and Pharmacy, Hue University, Hue, Vietnam
Tran Thi Van Thi
Faculty of Chemistry, Hue University of Sciences, Hue University, Hue, Vietnam
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Keywords

Myxopyrum smilacifolium
polysaccharides extraction
antioxidant activities

How to Cite

1.
Hieu LT, Le Lam S, Nguyen Dang Giang C, Nguyen Minh N, Tran Thanh M, Ho Xuan Anh V, Le Thuy T, Nguyen Viet T, Nguyen Quang M, Tran Thi Van T. Polysaccharide extraction from Myxopyrum smilacifolium trunk and its antioxidant capacity. hueuni-jns [Internet]. 2023Dec.30 [cited 2024Apr.27];132(1D):63-71. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7012
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Abstract

As a traditional medicinal plant in Vietnam, Myxopyrum smilacifolium has been used for a long history to treat cough, nervous disorders, numbness, rheumatism, and cephalalgia. Nevertheless, reports on the antioxidant activity and extraction of M. smilacifolium polysaccharides are still very rare. This study was designed to extract a high yield of polysaccharides from the M. smilacifolium trunk and characterize it. As a result, the maximum yield of the polysaccharides of 5.13 ± 0.05% was obtained with an extraction time of three hours, extraction temperature of 100 oC, the ratio of water to sample of 1:50 as extraction solution, extraction number of times 3, and the ratio of ethanol to extract volume 5:1 (v/v). Polysaccharides was characterized by Fourier transform infrared spectroscopy (FT-IR) and high-performance gel-permeation chromatography (HPGPC). The average molecular weight of extracted polysaccharides was around 3.78 × 105 Da. In vitro assays dan impressive antioxidant activities of the extracted polysaccharides, containing 0.2423 ± 0.0028 mg GA/g or 0.2142 ± 0.0007 μmol AS/g. The IC50 values of polysaccharides in the DPPH and ABTS methods were 0.89 mg/mL and 3.85 mg/mL, respectively.  These findings exhibited the potential for application or further research and development of polysaccharides from Myxopyrum smilacifolium.

https://doi.org/10.26459/hueunijns.v132i1D.7012
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