QuEChERS technique followed by GC-MS for trace detection of pesticides in vegetables and fruits
Vol. 132 No. 1A (2023), Original Article
Vol. 132 No. 1A (2023)

QuEChERS technique followed by GC-MS for trace detection of pesticides in vegetables and fruits

Original Article
https://doi.org/10.26459/hueunijns.v132i1A.7048
Published 2023-03-31
Huynh Thi Tuy Ngoc
Center for Testing Drugs, Cosmetics, and Foods, Thua Thien Hue Province, 17 Truong Dinh St., Hue, Vietnam
Nguyen Dang Giang Chau*
Faculty of Chemistry, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
PDF (Vietnamese)

Keywords

QuEChERS
pesticides
agricultural products
dispersive solid phase extraction QuEChERS
hóa chất bảo vệ thực vật
nông sản
chiết phân tán

How to Cite

1.
Huỳnh TTN, Châu N Đăng G. QuEChERS technique followed by GC-MS for trace detection of pesticides in vegetables and fruits. hueuni-jns [Internet]. 2023Mar.31 [cited 2024Dec.22];132(1A):111-2. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7048
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Abstract

Pesticide residues in agricultural products are an essential measure to assess food safety. In this study, we successfully developed and validated an effective analytical method for the simultaneous determination of nine new-generation pesticides in vegetables and fruits. We employed the QuEChERS sample preparation technique, using MgSO4 and sodium acetate trihydrate for the initial clean-up step, followed by dispersive solid-phase extraction with PSA and GCB adsorbents, to remove all impurities in the sample matrix. GC-MS was then utilized for qualitative and quantitative analysis. The method exhibited a low detection limit for analytes (3–8 µg·kg–1), a 70–120% recovery, good repeatability, and good linearity. We applied the technique in practice to analyze 16 vegetable and fruit samples collected at markets in Thua Thien Hue province and found that the residues of some pesticides were at high levels.

https://doi.org/10.26459/hueunijns.v132i1A.7048
PDF (Vietnamese)

References

  1. World Health Organization. Pesticide residues in food; 15 September 2022. Available from: https://www.who.int/news-room/fact-sheets/detail/pesticide-residues-in-food#:~:text=There%20are%20more%20than%201000,different%20properties%20and%20toxicological%20effects
  2. Ikpesu TO, Ariyo AB. Health Implication of Excessive Use and Abuse of Pesticides by the Rural Dwellers in Developing. Greener Journal of Environment Management and Public Safety. 2013;2(5):180-188.
  3. Alavanja MC, Hoppin JA, Kamel F. Health effects of chronic pesticide exposure: cancer and neurotoxicity. Annu Rev Public Health. 2004;25:155-97.
  4. Their HP, Zeumer H. In Manual of pesticide residue analysis Volume I. Germany: Wiley; 1987.
  5. Fenik J, Tankiewic M, Biziuk M. Properties and determination of pesticides in fruits and vegetables. TrAC – Trends in Analytical Chemistry. 2011; 30:814-826.
  6. Lozowicka B, Rutkowska E, Jankowska M. Influence of QuEChERS Modifications on Recovery and Matrix Effect during the Multi-Residue Pesticide Analysis in Soil by GC/MS/MS and GC/ECD/NPD. Environmental Science and Pollution Research. 2017;24:7124-7138.
  7. Alder L, Greulich K, Kempe K, Vieth B. Residue analysis of 500 high priority pesticides: better by GC-MS or LC-MS/MS?. Mass Spectrometry Reviews. 2006;25:838-865.
  8. Grimalt S, Dehouck P. Review of analytical methods for the determination of pesticide residues in grapes. J Chromatogr. A. 2016;1433:1-23.
  9. Philip LW, Meng CK. A method for the trace analysis of 175 pesticides using the Agilent triple quadrupole GC/MS/MS. Application note. Agilent Technologies, USA; 2009. Available from: https://www.wateronline.com/doc/a-method-for-the-trace-analysis-of-175-0002
  10. Anastassiades M, Lehotay SJ, Štajnbaher D, Schneck FJ. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce. Journal of AOAC International. 2003;86:412-431.
  11. Daniele O, Isabel CSFJ. Comparison of Different Sorbents in the QuEChERS Method for the Determination of Pesticide Residues in Strawberries by LC–MS/MS. Chromatographia. 2014;77:1291-1298.
  12. European Committee for Standardization EN 15662:2008. Foods of Plant Origin–Determination of Pesticide Residues Using GC–MS and/or LC–MS/MS Following Acetonitrile Extraction/Partitioning and Clean-up by Dispersive SPE–QuEChERS method. 2008.
  13. Miguel AGC, Diana AVM, Diego ARH. Pesticide-Residue Analysis in Soils by the QuEChERS Method: A Review. Molecules. 2022;27:4323.
  14. Phạm TL. Nghiên cứu phát triển kỹ thuật QuEChERS GC/MS 3 sim để phân tích đồng thời dư lượng hóa chất bảo vệ thực vật trong đất [dessertation]. Hà Nội: Viện Hàn lâm khoa học và công nghệ Việt Nam; 2013.
  15. AOAC International. AOAC Official Method 2007.01 Pesticide Residues in Foods by Acetonitrile Extraction and Partitioning with Magnesium Sulfate; 2007. Available from: https://nucleus.iaea.org/sites/fcris/Shared%20Documents/SOP/AOAC_2007_01.pdf
  16. European Commission. Guidance document on analytical quality control and method validation procedures for pesticide residues and analysis in food and feed, SANTE/11813/2017; 2017. Available from: https://ec.europa.eu/food/sites/food/files/plant/docs/pesticides_ mrl_guidelines_wrkdoc_2017-11813.pdf
  17. Klaudia P, Nasir S, Hanna B. A simple approach for pesticide residues determination in green vegetables based on QuEChERS and gas chromatography tandem mass spectrometry. Journal of Food Composition and Analysis. 2022;114:104783.
  18. Maciej T, Andrzej B. Improvement of the QuEChERS method coupled with GC–MS/MS for the determination of pesticide residues in fresh fruit and vegetables. Microchemical Journal. 2022; 181:107794.
  19. Seo YH, Cho TH, Hong CK, Kim MS, Cho SJ, Park WH, et al. Monitoring and risk assessment of pesticide residues in commercially dried vegetables. Preventive Nutrition and Food Science. 2013; 18(2):145-149.
  20. Nguyen DGC C, Le DBC, Nguyen VH, Hoang TL, Tran TVT, Huynh TPL. Assessment of pesticide use and pesticide residues in vegetables from two provinces in Central Vietnam. PLoS ONE. 2022; 17(6): e0269789.
  21. Kortenkamp A, Backhaus T, Faust M. State of the art report on mixture toxicity. European Commission 070307/2007/485103/ETU/D.1; 2009.
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