Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector
Vol. 132 No. 1D (2023), Original Article
Vol. 132 No. 1D (2023)

Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector

Original Article
https://doi.org/10.26459/hueunijns.v132i1D.7075
Published 2023-12-30
Nguyen Hoang Tue
Institute of Bioactive Compounds (IBT), University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet Nam
Nguyen Ngoc Luong
Institute of Bioactive Compounds (IBT), University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet Nam
Nguyen Quang Duc Tien*
Institute of Bioactive Compounds (IBT), University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet Nam
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Keywords

SARS-CoV-2 antigen
RBD
C5A
T4
Cloning

How to Cite

1.
Tue NH, Luong NN, Tien NQD. Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector. hueuni-jns [Internet]. 2023Dec.30 [cited 2024Apr.28];132(1D):73-8. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7075
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Abstract

This study aims to make a plant expression vector with an RBD-T4-Linker-C5a sequence that codes for the SARS-CoV-2 antigen and an A3Dsp signal peptide from the rice 3D amylase gene located before the RBD. Methods of molecular cloning were applied in this study. The plant expression vector pNHL22 harboring the RBD-T4-Linker-C5a sequence was successfully established and conjugated into Agrobacterium tumefaciens LBA4404 by triparental mating. Bacteria A. tumefaciens containing the RBD-T4-Linker-C5a sequence are now ready for genetic transformation into the Nicotiana benthamiana plant for future applications.

https://doi.org/10.26459/hueunijns.v132i1D.7075
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